Comparative evaluation of antifungal efficacy of conventional endodontic irrigants and chitosan nanoparticles

• Published in: Journal of conservative dentistry 2023-03, Vol.26 (2), p.226-229
• Main Authors: Balsaraf, Omkar, Raghavendra, Srinidhi, Shah, Dipali, Sanjyot, Mulay, Balsaraf, Aishwarya
• Format: Article
• Language: English
• Subjects: Analysis; Antifungal activity; Antifungal agents; Chitosan; Chlorhexidine; Dextrose; Disc-diffusion test; Nanoparticles; Original; Sodium hypochlorite; Statistical analysis
• ISSN: 0972-0707; 0974-5203
• DOI: 10.4103/jcd.jcd_617_22

Aim: The purpose of this study was to evaluate the efficacy of 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and chitosan nanoparticles against Candida albicans using the agar disc-diffusion test. Materials and Methods: Strain of C. albicans was cultivated in Sabouraud Dextrose Agar. Chitosan nanoparticles were synthesized using an ionic gelation method. Four groups were made according to the irrigants used. Group 1: 3% NaOCl, Group 2: 2% CHX, Group 3: chitosan nanoparticles, and Group 4: saline as control. Discs were added with the different irrigants and placed in a dish containing C. albicans. The plates were incubated at 37°C for 24h. The zone of inhibition was measured in millimeter. Results: Statistical analysis was performed using the test of one-way variance (ANOVA) and post hoc Tukey. Group 1 showed significantly higher zone of inhibition compared to Groups 2 and 3 (P < 0.05). There were no significant differences between the zones of inhibition of Groups 2 and 3 (P < 0.05). Conclusion: Chitosan nanoparticles and 2% CHX have similar efficacy against C. albicans, whereas 3% NaOCl was significantly better than both chitosan nanoparticles and CHX.