Label-free intratissue activity imaging of alveolar organoids with dynamic optical coherence tomography

An organoid is a three-dimensional (3D) cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem cells (hiPSCs)-derived alveolar organoids in normal and fibrosis models....

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Bibliographic Details
Published in:Biomedical optics express 2023-05, Vol.14 (5), p.2333-2351
Main Authors: Morishita, Rion, Suzuki, Toshio, Mukherjee, Pradipta, Abd El-Sadek, Ibrahim, Lim, Yiheng, Lichtenegger, Antonia, Makita, Shuichi, Tomita, Kiriko, Yamamoto, Yuki, Nagamoto, Tetsuharu, Yasuno, Yoshiaki
Format: Article
Language:English
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Summary:An organoid is a three-dimensional (3D) cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem cells (hiPSCs)-derived alveolar organoids in normal and fibrosis models. 3D DOCT data were acquired with an 840-nm spectral domain optical coherence tomography with axial and lateral resolutions of 3.8 µm (in tissue) and 4.9 µm, respectively. The DOCT images were obtained by the logarithmic-intensity-variance (LIV) algorithm, which is sensitive to the signal fluctuation magnitude. The LIV images revealed cystic structures surrounded by high-LIV borders and mesh-like structures with low LIV. The former may be alveoli with a highly dynamics epithelium, while the latter may be fibroblasts. The LIV images also demonstrated the abnormal repair of the alveolar epithelium.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.488097