The sulfur-regulated arylsulfatase gene cluster of Pseudomonas aeruginosa, a new member of the cys regulon

A gene cluster upstream of the arylsulfatase gene (atsA) in Pseudomonas aeruginosa was characterized and found to encode a putative ABC-type transporter, AtsRBC. Mutants with insertions in the atsR or atsB gene were unable to grow with hexyl-, octyl-, or nitrocatecholsulfate, although they grew norm...

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Bibliographic Details
Published in:Journal of bacteriology 2000-04, Vol.182 (7), p.2055-2058
Main Authors: Hummerjohann, J, Laudenbach, S, Rétey, J, Leisinger, T, Kertesz, M A
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Arylsulfatases - genetics
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Bacteriology
Biological Transport - genetics
Cloning, Molecular
Esters - metabolism
Gene Expression Regulation, Bacterial - drug effects
Genes
Genes, Bacterial - genetics
Genes, Bacterial - physiology
Genetics and Molecular Biology
Molecular Sequence Data
Multigene Family
Mutation - genetics
Pseudomonas aeruginosa
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - growth & development
Pseudomonas aeruginosa - metabolism
Regulon - genetics
Sulfates - metabolism
Sulfur
Sulfur - metabolism
Sulfur - pharmacology
Trans-Activators - genetics
Trans-Activators - physiology
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Summary:A gene cluster upstream of the arylsulfatase gene (atsA) in Pseudomonas aeruginosa was characterized and found to encode a putative ABC-type transporter, AtsRBC. Mutants with insertions in the atsR or atsB gene were unable to grow with hexyl-, octyl-, or nitrocatecholsulfate, although they grew normally with other sulfur sources, such as sulfate, methionine, and aliphatic sulfonates. AtsRBC therefore constitutes a general sulfate ester transport system, and desulfurization of aromatic and medium-chain-length aliphatic sulfate esters occurs in the cytoplasm. Expression of the atsR and atsBCA genes was repressed during growth with sulfate, cysteine, or thiocyanate. No expression of these genes was observed in the cysB mutant PAO-CB, and the ats genes therefore constitute an extension of the cys regulon in this species.
ISSN:0021-9193
1098-5530
DOI:10.1128/JB.182.7.2055-2058.2000