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Cuproptosis-Related Genes CDK1 and COA6 Involved in the Prognosis Prediction of Liver Hepatocellular Carcinoma

Background. Liver hepatocellular carcinoma (LIHC) is the most frequently seen type of primary liver cancer. Cuproptosis is a novel form of cell death highly associated with mitochondrial metabolism. However, the clinical impact and pertinent mechanism of cuproptosis genes in LIHC remain largely unkn...

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Published in:Disease markers 2023, Vol.2023, p.5552798-17
Main Authors: Han, Sanfeng, Ye, Tao, Mao, Yuqin, Hu, Bo, Wang, Chen
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Mao, Yuqin
Hu, Bo
Wang, Chen
description Background. Liver hepatocellular carcinoma (LIHC) is the most frequently seen type of primary liver cancer. Cuproptosis is a novel form of cell death highly associated with mitochondrial metabolism. However, the clinical impact and pertinent mechanism of cuproptosis genes in LIHC remain largely unknown. Methods. From public databases, we systematically assessed common genes from LIHC differentially expressed genes (DEGs) and cuproptosis-related genes using bioinformatics analysis. These common genes were then analyzed by enrichment analysis, mutation analysis, risk score model, and others to find candidate hub genes related to LIHC and cuproptosis. Next, hub genes were determined by expression, clinical factors, immunoassay, and prognostic nomogram. Results. Based on 129 cuproptosis-related genes and 3492 LIHC DEGs, we totally identified 21 downregulated and 18 upregulated common genes, and they were enriched in pathways, such as zinc ion homeostasis and oxidative phosphorylation. In the mutation analysis, missense mutation was the most common type in LIHC patients, and the common gene F5 had the highest mutation frequency. After LASSO-Cox regression analysis and prognostic analysis, CDK1, ABCB6, LCAT, and COA6 were identified as prognostic signature genes. Among them, ABCB6 and LCAT were lowly expressed in tumors, and CDK1 and COA6 were highly expressed in tumors. In addition, ABCB6 and LCAT were negatively correlated with 6 kinds of immune cells, while CDK1 and COA6 were positively correlated with them. CDK1 and COA6 were identified as hub genes related to LIHC by Cox regression analysis and prognostic nomogram. Conclusion. CDK1 and COA6 are two oncogenes in LIHC, which are involved in the molecular mechanism of cuproptosis and LIHC. Besides, CDK1 and COA6 can positively regulate the expressions of immune cells in LIHC. In clinical practice, they can be used as immunotherapeutic targets and prognostic predictors in LIHC, which sheds new light on the scientific fields of cuproptosis and LIHC.
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Liver hepatocellular carcinoma (LIHC) is the most frequently seen type of primary liver cancer. Cuproptosis is a novel form of cell death highly associated with mitochondrial metabolism. However, the clinical impact and pertinent mechanism of cuproptosis genes in LIHC remain largely unknown. Methods. From public databases, we systematically assessed common genes from LIHC differentially expressed genes (DEGs) and cuproptosis-related genes using bioinformatics analysis. These common genes were then analyzed by enrichment analysis, mutation analysis, risk score model, and others to find candidate hub genes related to LIHC and cuproptosis. Next, hub genes were determined by expression, clinical factors, immunoassay, and prognostic nomogram. Results. Based on 129 cuproptosis-related genes and 3492 LIHC DEGs, we totally identified 21 downregulated and 18 upregulated common genes, and they were enriched in pathways, such as zinc ion homeostasis and oxidative phosphorylation. In the mutation analysis, missense mutation was the most common type in LIHC patients, and the common gene F5 had the highest mutation frequency. After LASSO-Cox regression analysis and prognostic analysis, CDK1, ABCB6, LCAT, and COA6 were identified as prognostic signature genes. Among them, ABCB6 and LCAT were lowly expressed in tumors, and CDK1 and COA6 were highly expressed in tumors. In addition, ABCB6 and LCAT were negatively correlated with 6 kinds of immune cells, while CDK1 and COA6 were positively correlated with them. CDK1 and COA6 were identified as hub genes related to LIHC by Cox regression analysis and prognostic nomogram. Conclusion. CDK1 and COA6 are two oncogenes in LIHC, which are involved in the molecular mechanism of cuproptosis and LIHC. Besides, CDK1 and COA6 can positively regulate the expressions of immune cells in LIHC. In clinical practice, they can be used as immunotherapeutic targets and prognostic predictors in LIHC, which sheds new light on the scientific fields of cuproptosis and LIHC.</description><identifier>ISSN: 0278-0240</identifier><identifier>EISSN: 1875-8630</identifier><identifier>DOI: 10.1155/2023/5552798</identifier><identifier>PMID: 37215201</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Apoptosis ; Bioinformatics ; Biomarkers ; Cancer therapies ; Carcinoma, Hepatocellular - genetics ; Carrier Proteins ; CDC2 Protein Kinase - genetics ; Cell death ; Cholangiocarcinoma ; Copper ; Gene expression ; Genes ; Hepatocellular carcinoma ; Homeostasis ; Humans ; Immune system ; Immunoassay ; Liver ; Liver cancer ; Liver Neoplasms - genetics ; Medical prognosis ; Melanoma ; Metabolism ; Missense mutation ; Mitochondria ; Mitochondrial Proteins ; Molecular modelling ; Mutation ; Nomograms ; Oxidative phosphorylation ; Phosphorylation ; Prognosis ; Regression analysis ; Risk analysis ; Software ; Survival analysis ; Tumors</subject><ispartof>Disease markers, 2023, Vol.2023, p.5552798-17</ispartof><rights>Copyright © 2023 Sanfeng Han et al.</rights><rights>Copyright © 2023 Sanfeng Han et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2023 Sanfeng Han et al. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3648-10bfde82949518e640dac5c3b0966944b05e85141131f898887b04798281dc583</citedby><cites>FETCH-LOGICAL-c3648-10bfde82949518e640dac5c3b0966944b05e85141131f898887b04798281dc583</cites><orcidid>0000-0001-6348-010X ; 0009-0008-1676-3623</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37215201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wang, Tian jiao</contributor><contributor>Tian jiao Wang</contributor><creatorcontrib>Han, Sanfeng</creatorcontrib><creatorcontrib>Ye, Tao</creatorcontrib><creatorcontrib>Mao, Yuqin</creatorcontrib><creatorcontrib>Hu, Bo</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><title>Cuproptosis-Related Genes CDK1 and COA6 Involved in the Prognosis Prediction of Liver Hepatocellular Carcinoma</title><title>Disease markers</title><addtitle>Dis Markers</addtitle><description>Background. Liver hepatocellular carcinoma (LIHC) is the most frequently seen type of primary liver cancer. Cuproptosis is a novel form of cell death highly associated with mitochondrial metabolism. However, the clinical impact and pertinent mechanism of cuproptosis genes in LIHC remain largely unknown. Methods. From public databases, we systematically assessed common genes from LIHC differentially expressed genes (DEGs) and cuproptosis-related genes using bioinformatics analysis. These common genes were then analyzed by enrichment analysis, mutation analysis, risk score model, and others to find candidate hub genes related to LIHC and cuproptosis. Next, hub genes were determined by expression, clinical factors, immunoassay, and prognostic nomogram. Results. Based on 129 cuproptosis-related genes and 3492 LIHC DEGs, we totally identified 21 downregulated and 18 upregulated common genes, and they were enriched in pathways, such as zinc ion homeostasis and oxidative phosphorylation. In the mutation analysis, missense mutation was the most common type in LIHC patients, and the common gene F5 had the highest mutation frequency. After LASSO-Cox regression analysis and prognostic analysis, CDK1, ABCB6, LCAT, and COA6 were identified as prognostic signature genes. Among them, ABCB6 and LCAT were lowly expressed in tumors, and CDK1 and COA6 were highly expressed in tumors. In addition, ABCB6 and LCAT were negatively correlated with 6 kinds of immune cells, while CDK1 and COA6 were positively correlated with them. CDK1 and COA6 were identified as hub genes related to LIHC by Cox regression analysis and prognostic nomogram. Conclusion. CDK1 and COA6 are two oncogenes in LIHC, which are involved in the molecular mechanism of cuproptosis and LIHC. Besides, CDK1 and COA6 can positively regulate the expressions of immune cells in LIHC. 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Ye, Tao ; Mao, Yuqin ; Hu, Bo ; Wang, Chen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3648-10bfde82949518e640dac5c3b0966944b05e85141131f898887b04798281dc583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Apoptosis</topic><topic>Bioinformatics</topic><topic>Biomarkers</topic><topic>Cancer therapies</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carrier Proteins</topic><topic>CDC2 Protein Kinase - genetics</topic><topic>Cell death</topic><topic>Cholangiocarcinoma</topic><topic>Copper</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Hepatocellular carcinoma</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunoassay</topic><topic>Liver</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Medical prognosis</topic><topic>Melanoma</topic><topic>Metabolism</topic><topic>Missense mutation</topic><topic>Mitochondria</topic><topic>Mitochondrial Proteins</topic><topic>Molecular modelling</topic><topic>Mutation</topic><topic>Nomograms</topic><topic>Oxidative phosphorylation</topic><topic>Phosphorylation</topic><topic>Prognosis</topic><topic>Regression analysis</topic><topic>Risk analysis</topic><topic>Software</topic><topic>Survival analysis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Sanfeng</creatorcontrib><creatorcontrib>Ye, Tao</creatorcontrib><creatorcontrib>Mao, Yuqin</creatorcontrib><creatorcontrib>Hu, Bo</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Sanfeng</au><au>Ye, Tao</au><au>Mao, Yuqin</au><au>Hu, Bo</au><au>Wang, Chen</au><au>Wang, Tian jiao</au><au>Tian jiao Wang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cuproptosis-Related Genes CDK1 and COA6 Involved in the Prognosis Prediction of Liver Hepatocellular Carcinoma</atitle><jtitle>Disease markers</jtitle><addtitle>Dis Markers</addtitle><date>2023</date><risdate>2023</risdate><volume>2023</volume><spage>5552798</spage><epage>17</epage><pages>5552798-17</pages><issn>0278-0240</issn><eissn>1875-8630</eissn><abstract>Background. Liver hepatocellular carcinoma (LIHC) is the most frequently seen type of primary liver cancer. Cuproptosis is a novel form of cell death highly associated with mitochondrial metabolism. However, the clinical impact and pertinent mechanism of cuproptosis genes in LIHC remain largely unknown. Methods. From public databases, we systematically assessed common genes from LIHC differentially expressed genes (DEGs) and cuproptosis-related genes using bioinformatics analysis. These common genes were then analyzed by enrichment analysis, mutation analysis, risk score model, and others to find candidate hub genes related to LIHC and cuproptosis. Next, hub genes were determined by expression, clinical factors, immunoassay, and prognostic nomogram. Results. Based on 129 cuproptosis-related genes and 3492 LIHC DEGs, we totally identified 21 downregulated and 18 upregulated common genes, and they were enriched in pathways, such as zinc ion homeostasis and oxidative phosphorylation. In the mutation analysis, missense mutation was the most common type in LIHC patients, and the common gene F5 had the highest mutation frequency. After LASSO-Cox regression analysis and prognostic analysis, CDK1, ABCB6, LCAT, and COA6 were identified as prognostic signature genes. Among them, ABCB6 and LCAT were lowly expressed in tumors, and CDK1 and COA6 were highly expressed in tumors. In addition, ABCB6 and LCAT were negatively correlated with 6 kinds of immune cells, while CDK1 and COA6 were positively correlated with them. CDK1 and COA6 were identified as hub genes related to LIHC by Cox regression analysis and prognostic nomogram. Conclusion. CDK1 and COA6 are two oncogenes in LIHC, which are involved in the molecular mechanism of cuproptosis and LIHC. Besides, CDK1 and COA6 can positively regulate the expressions of immune cells in LIHC. In clinical practice, they can be used as immunotherapeutic targets and prognostic predictors in LIHC, which sheds new light on the scientific fields of cuproptosis and LIHC.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>37215201</pmid><doi>10.1155/2023/5552798</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0001-6348-010X</orcidid><orcidid>https://orcid.org/0009-0008-1676-3623</orcidid><oa>free_for_read</oa></addata></record>
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subjects Apoptosis
Bioinformatics
Biomarkers
Cancer therapies
Carcinoma, Hepatocellular - genetics
Carrier Proteins
CDC2 Protein Kinase - genetics
Cell death
Cholangiocarcinoma
Copper
Gene expression
Genes
Hepatocellular carcinoma
Homeostasis
Humans
Immune system
Immunoassay
Liver
Liver cancer
Liver Neoplasms - genetics
Medical prognosis
Melanoma
Metabolism
Missense mutation
Mitochondria
Mitochondrial Proteins
Molecular modelling
Mutation
Nomograms
Oxidative phosphorylation
Phosphorylation
Prognosis
Regression analysis
Risk analysis
Software
Survival analysis
Tumors
title Cuproptosis-Related Genes CDK1 and COA6 Involved in the Prognosis Prediction of Liver Hepatocellular Carcinoma
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