Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells

We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and...

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Bibliographic Details
Published in:Journal of bacteriology 2000-05, Vol.182 (10), p.2680-2686
Main Authors: Tolker-Nielsen, T, Christensen, A B, Holmstrøm, K, Eberl, L, Rasmussen, T B, Sternberg, C, Heydorn, A, Molin, S, Givskov, M
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Bacteriology
Cells
DNA-Binding Proteins - analysis
DNA-Binding Proteins - genetics
Escherichia coli Proteins
flhDC gene
Gene Expression
Gene Expression Regulation, Bacterial - drug effects
Genes
Isopropyl Thiogalactoside - pharmacology
Microbiology
Operon
Pathogens
Physiology and Metabolism
Recombinant Fusion Proteins - genetics
Ribonucleic acid
RNA
RNA, Bacterial - analysis
RNA, Messenger - analysis
Serratia - drug effects
Serratia - genetics
Serratia - growth & development
Serratia liquefaciens
Trans-Activators - analysis
Trans-Activators - genetics
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Summary:We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.
ISSN:0021-9193
1098-5530
DOI:10.1128/JB.182.10.2680-2686.2000