Separation and Detection of Tyrosine and Phenylalanine‐derived Oxidative Stress Biomarkers Using Microchip Electrophoresis with Electrochemical Detection

A method for the determination of selected aromatic amino acid biomarkers of oxidative stress using microchip electrophoresis with electrochemical detection is described. The separation of the major reaction products of phenylalanine and tyrosine with reactive nitrogen and oxygen species was accompl...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2022-12, Vol.34 (12), p.1913-1927
Main Authors: Weerasekara, Dhanushka B., Lunte, Susan M.
Format: Article
Language:English
Subjects:
hydroxyl radical
microchip electrophoresis with electrochemical detection
peroxynitrite
reactive nitrogen and oxygen species
tyrosine
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Summary:A method for the determination of selected aromatic amino acid biomarkers of oxidative stress using microchip electrophoresis with electrochemical detection is described. The separation of the major reaction products of phenylalanine and tyrosine with reactive nitrogen and oxygen species was accomplished using ligand exchange micellar electrokinetic chromatography with a PDMS/glass hybrid chip. Electrochemical detection was achieved using a pyrolyzed photoresist film working electrode. The system was evaluated for the analysis of the products of the Fenton reaction with tyrosine and phenylalanine, and the reaction of peroxynitrite with tyrosine.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.202100580