Addendum: Systems vaccinology of the BNT162b2 mRNA vaccine in humans

• Published in: Nature (London) 2023-06, Vol.618 (7964), p.E18-E18
• Main Authors: Arunachalam, Prabhu S, Scott, Madeleine K D, Hagan, Thomas, Li, Chunfeng, Feng, Yupeng, Wimmers, Florian, Grigoryan, Lilit, Trisal, Meera, Edara, Venkata Viswanadh, Lai, Lilin, Chang, Sarah Esther, Feng, Allan, Dhingra, Shaurya, Shah, Mihir, Lee, Alexandra S, Chinthrajah, Sharon, Sindher, Sayantani B, Mallajosyula, Vamsee, Gao, Fei, Sigal, Natalia, Kowli, Sangeeta, Gupta, Sheena, Pellegrini, Kathryn, Tharp, Gregory, Maysel-Auslender, Sofia, Hamilton, Sydney, Aoued, Hadj, Hrusovsky, Kevin, Roskey, Mark, Bosinger, Steven E, Maecker, Holden T, Boyd, Scott D, Davis, Mark M, Utz, Paul J, Suthar, Mehul S, Khatri, Purvesh, Nadeau, Kari C, Pulendran, Bali
• Format: Article
• Language: English
• Subjects: Addendum; BNT162 Vaccine; Humans; mRNA Vaccines; Neutralization; Vaccines, Synthetic; Vaccinology
• ISSN: 0028-0836; 1476-4687; 1476-4687
• DOI: 10.1038/s41586-023-05977-x

[...]there was a cross-contamination of the day-1 samples obtained from participants 2049 (male) and 2051 (female), which resulted in the erroneous appearance of cell clusters containing cells of both male and female origin in the CITE-seq data. The major conclusions from the analysis of bulk transcriptional data, including the dynamics and nature of the transcriptional response, and the transcriptional correlates of T cell and antibody response remain unaffected. [...]the reassignment ofsamples to the appropriate time points resulted in more negatively enri1ched gene sets on day 7 and later, and a diminution of the correlation between the monocyte-associated transcriptional signature and the cross-neutralization potential against the B.1.351 strain (Extended Data Fig. 12).