An aggregation of human embryonic and trophoblast stem cells reveals the role of trophectoderm on epiblast differentiation

The interactions between extra‐embryonic tissues and embryonic tissues are crucial to ensure proper early embryo development. However, the understanding of the crosstalk between the embryonic tissues and extra‐embryonic tissues is lacking, mainly due to ethical restrictions, difficulties in obtainin...

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Bibliographic Details
Published in:Cell proliferation 2023-05, Vol.56 (5), p.e13492-n/a
Main Authors: Wu, Xulun, Zhao, Wentao, Wu, Hao, Zhang, Qiancheng, Wang, Yiming, Yu, Kunyuan, Zhai, Jinglei, Mo, Fan, Wang, Meijiao, Li, Shiwen, Zhu, Xili, Liang, Xiaoyan, Hu, Baoyang, Liu, Guang‐Hui, Wu, Jun, Wang, Hongmei, Guo, Fan, Yu, Leqian
Format: Article
Language:English
Subjects:
Aggregates
Antibodies
Asymmetric structures
Cell Differentiation
Embryo cells
Embryogenesis
Embryos
Gastrula - physiology
Gastrulation
Germ Layers
Humans
Lasers
Medical research
Mesoderm
Original
Primitive streak
Stem Cells
Trophectoderm
Trophoblasts
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Summary:The interactions between extra‐embryonic tissues and embryonic tissues are crucial to ensure proper early embryo development. However, the understanding of the crosstalk between the embryonic tissues and extra‐embryonic tissues is lacking, mainly due to ethical restrictions, difficulties in obtaining natural human embryos, and lack of appropriate in vitro models. Here by aggregating human embryonic stem cells (hESCs) with human trophoblast stem cells (hTSCs), we revealed the hESCs robustly self‐organized into a unique asymmetric structure which the primitive streak (PS) like cells exclusively distributed at the distal end to the TS‐compartment, and morphologically flattened cells, presumed to be the extra‐embryonic mesoderm cells (EXMC) like cells, were induced at the proximal end to hTSCs. Our study revealed two potential roles of extra‐embryonic trophectoderm in regulating the proper PS formation during gastrulation and EXMCs induction from the human epiblast. In this study, an in vitro model (ET‐aggregates, ETAs) was constructed by aggregating human embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) to investigate the crosstalk between human trophectoderm and epiblast. The ES‐compartment of ETAs showed the lumenogenesis and robustly self‐organized into a unique asymmetric structure in which the most of primitive streak‐like cells distributed at the distal end to the TS‐compartment, and morphologically flattened cells, presumed to be the extra‐embryonic mesoderm cells (EXMCs), were induced at the proximal end to hTSCs.
ISSN:0960-7722
1365-2184
DOI:10.1111/cpr.13492