P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation

p53 immunohistochemistry (IHC) has been proposed as a surrogate for mutations in penile squamous cell carcinomas (PSCC). We aimed to evaluate the performance of a pattern-based evaluation of p53 IHC in PSCC. Human papilloma virus (HPV) DNA testing, p16 and p53 IHC, and whole exome sequencing were pe...

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Bibliographic Details
Published in:Cancers 2023-05, Vol.15 (10), p.2719
Main Authors: Trias, Isabel, Saco, Adela, Marimon, Lorena, López Del Campo, Ricardo, Manzotti, Carolina, Ordi, Oriol, Del Pino, Marta, Pérez, Francisco M, Vega, Naiara, Alós, Silvia, Martínez, Antonio, Rodriguez-Carunchio, Leonardo, Reig, Oscar, Jares, Pedro, Teixido, Cristina, Ajami, Tarek, Corral-Molina, Juan Manuel, Algaba, Ferran, Ribal, María J, Ribera-Cortada, Inmaculada, Rakislova, Natalia
Format: Article
Language:English
Subjects:
Analysis
Cancer
Cell cycle
DNA viruses
Genetic aspects
Genomes
Human papillomavirus
Immunohistochemistry
Lymph nodes
Lymphatic system
Medical laboratories
Metastases
Mutation
p53 Protein
Papillomavirus infections
Penis
Prognosis
Proteins
Risk groups
Squamous cell carcinoma
Tumor proteins
Tumors
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Summary:p53 immunohistochemistry (IHC) has been proposed as a surrogate for mutations in penile squamous cell carcinomas (PSCC). We aimed to evaluate the performance of a pattern-based evaluation of p53 IHC in PSCC. Human papilloma virus (HPV) DNA testing, p16 and p53 IHC, and whole exome sequencing were performed in a series of 40 PSCC. p53 IHC was evaluated following a pattern-based framework and conventional p53 IHC evaluation. Out of 40 PSCC, 12 (30.0%) were HPV-associated, and 28 (70.0%) were HPV-independent. The agreement between the p53 IHC pattern-based evaluation and mutational status was almost perfect (k = 0.85). The sensitivity and accuracy of the pattern-based framework for identifying mutations were 95.5% and 92.5%, respectively, which were higher than the values of conventional p53 IHC interpretation (54.5% and 70.0%, respectively), whereas the specificity was the same (88.9%). In conclusions, the pattern-based framework improves the accuracy of detecting mutations in PSCC compared to the classical p53 IHC evaluation.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers15102719