Innovative Rocuronium Bromide Topical Formulation for Targeted Skin Drug Delivery: Design, Comprehensive Characterization, In Vitro 2D/3D Human Cell Culture and Permeation

Cutaneous squamous cell carcinoma (cSCC) is the second-most common type of non-melanoma skin cancer and is linked to long-term exposure to ultraviolet (UV) radiation from the sun. Rocuronium bromide (RocBr) is an FDA-approved drug that targets p53-related protein kinase (PRPK) that inhibits the deve...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-05, Vol.24 (10), p.8776
Main Authors: Ruiz, Victor H, Encinas-Basurto, David, Sun, Bo, Eedara, Basanth Babu, Roh, Eunmiri, Alarcon, Neftali Ortega, Curiel-Lewandrowski, Clara, Bode, Ann M, Mansour, Heidi M
Format: Article
Language:English
Subjects:
Biomimetics
Cancer therapies
Carcinoma, Squamous Cell - pathology
Cell culture
Cell Culture Techniques
Cells
Dermatologic agents
Dermatology
Drug delivery
Drug development
Electron microscopy
Formulae, receipts, prescriptions
Humans
Kinases
Melanoma
Membranes
p53 Protein
Pharmaceutical Preparations - metabolism
Physicochemical properties
Rocuronium - pharmacology
Rocuronium bromide
Scanning electron microscopy
Skin
Skin - metabolism
Skin cancer
Skin Neoplasms - pathology
Spectroscopy
Spectrum analysis
Squamous cell carcinoma
Thermal analysis
Transdermal medication
Ultraviolet radiation
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Summary:Cutaneous squamous cell carcinoma (cSCC) is the second-most common type of non-melanoma skin cancer and is linked to long-term exposure to ultraviolet (UV) radiation from the sun. Rocuronium bromide (RocBr) is an FDA-approved drug that targets p53-related protein kinase (PRPK) that inhibits the development of UV-induced cSCC. This study aimed to investigate the physicochemical properties and in vitro behavior of RocBr. Techniques such as thermal analysis, electron microscopy, spectroscopy and in vitro assays were used to characterize RocBr. A topical oil/water emulsion lotion formulation of RocBr was successfully developed and evaluated. The in vitro permeation behavior of RocBr from its lotion formulation was quantified with Strat-M synthetic biomimetic membrane and EpiDerm™ 3D human skin tissue. Significant membrane retention of RocBr drug was evident and more retention was obtained with the lotion formulation compared with the solution. This is the first systematic and comprehensive study to report these findings.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24108776