Gonadotropins and Sex Steroid Hormones in Captive-Reared Small Yellow Croaker ( Larimichthys polyactis ) and Their Role in Female Reproductive Dysfunction

• Published in: International journal of molecular sciences 2023-05, Vol.24 (10), p.8919
• Main Authors: Kho, Kang Hee, Sukhan, Zahid Parvez, Yang, Seok-Woo, Hwang, Nam-Yong, Lee, Won-Kyo
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Animals; Endocrine system; Enzyme-linked immunosorbent assay; Estradiol; Estradiol - metabolism; Estradiol - pharmacology; Female; Females; Fish; Fishes; Fishing; Follicle Stimulating Hormone, beta Subunit - metabolism; Follicle-stimulating hormone; Glycoproteins; Gonadal Steroid Hormones - metabolism; Gonadotropins; Hormones; Luteinizing hormone; Luteinizing Hormone, beta Subunit; Male; Males; Negative feedback; Ovaries; Perciformes; Pituitary; Pituitary (anterior); Pituitary gland; Pituitary Gland - metabolism; Progesterone; Sex hormones; Spawning; Spermatogenesis; Steroids; Steroids - metabolism; Testosterone
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24108919

The seed production of small yellow croaker (SYC) is constrained by reproductive dysfunction in captive-reared females. Reproductive dysfunction is closely linked to endocrine reproductive mechanisms. To better understand the reproductive dysfunction in captive broodstock, functional characterization of gonadotropins (GtHs: follicle stimulating hormone β subunit, luteinizing hormone β subunit, and glycoprotein α subunit, ) and sex steroids (17β-estradiol, E2; testosterone, T; progesterone; P) was performed using qRT-PCR, ELISA, in vivo, and in-vitro assay. The pituitary GtHs and gonadal steroids levels were significantly higher in ripen fish of both sexes. However, changes in and E2 levels in females were not significant in the developing and ripen stages. Furthermore, GtHs and steroids levels were lower in females compared to males throughout the reproductive cycle. In vivo administration of gonadotropin releasing hormone analogue (GnRHa) significantly increased the expression of GtHs in both dose- and time-related manners. The lower and higher doses of GnRHa led to successful spawning in male and female SYC, respectively. Sex steroids in vitro significantly inhibited the expression of in female SYC. Overall, GtHs were shown to play a vital role in final gonadal maturation, while steroids promoted negative feedback in the regulation of pituitary GtHs. Lower levels of GtHs and steroids might be key components in the reproductive dysfunction of captive-reared female SYC.