Genome transfer technique for bovine embryo production using the metaphase plate and polar body

Despite many studies in humans and mice using genome transfer (GT), there are few reports using this technique in oocytes of wild or domestic animals. Therefore, we aimed to establish a GT technique in bovine oocytes using the metaphase plate (MP) and polar body (PB) as the sources of genetic materi...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2023-04, Vol.40 (4), p.943-951
Main Authors: Dode, M. A. N., Caixeta, F. M. C., Vargas, L. N., Leme, L. O., Kawamoto, T. S., Fidelis, A. A. G., Franco, M. M.
Format: Article
Language:English
Subjects:
Animals
Blastocyst
Cattle
Cryopreservation - methods
Domestic animals
Embryo Biology
Embryos
Fertilization
Fertilization in Vitro - methods
Genomes
Gynecology
Human Genetics
Humans
Male
Medicine
Medicine & Public Health
Metaphase
Metaphase - genetics
Mice
Mitochondrial DNA
Oocytes
Polar Bodies
Reproductive Medicine
Semen
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Summary:Despite many studies in humans and mice using genome transfer (GT), there are few reports using this technique in oocytes of wild or domestic animals. Therefore, we aimed to establish a GT technique in bovine oocytes using the metaphase plate (MP) and polar body (PB) as the sources of genetic material. In the first experiment, GT was established using MP (GT-MP), and a sperm concentration of 1 × 10 6 or 0.5 × 10 6 spermatozoa/ml gave similar fertilization rates. The cleavage rate (50%) and blastocyst rate (13.6%) in the GT-MP group was lower than that of the in vitro production control group (80.2% and 32.6%, respectively). The second experiment evaluated the same parameters using PB instead of MP; the GT-PB group had lower fertilization (82.3% vs. 96.2%) and blastocyst (7.7% vs. 36.8%) rates than the control group. No differences in the amount of mitochondrial DNA (mtDNA) were observed between groups. Finally, GT-MP was performed using vitrified oocytes (GT-MPV) as a source of genetic material. The cleavage rate of the GT-MPV group (68.4%) was similar to that of the vitrified oocytes (VIT) control group (70.0%) and to that of the control IVP group (81.25%, P < 0.05). The blastocyst rate of GT-MPV (15.7) did not differ neither from the VIT control group (5.0%) nor from the IVP control group (35.7%). The results suggested that the structures reconstructed by the GT-MPV and GT-PB technique develop in embryos even if vitrified oocytes are used.
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-023-02758-3