Bacteria and antimicrobial resistance profile during the composting process of wastes from animal production

• Published in: Brazilian journal of microbiology 2023-06, Vol.54 (2), p.1157-1167
• Main Authors: Ferreira, Paula Fernanda Alves, Xavier, Júlia Ferreira, Nunes, Juliana Ferreira, Fonseca, Isabela Pinto, de Mattos de Oliveira Coelho, Shana, Soares de Souza, Miliane Moreira, da Silva Coelho, Irene
• Format: Article
• Language: English
• Subjects: Agricultural runoff; Agricultural wastes; Agriculture; Animal production; Animal wastes; Animals; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Antimicrobial resistance; Bacteria; Bedding; Biomedical and Life Sciences; Composting; Contaminants; Drug resistance; Drug Resistance, Bacterial; E coli; Escherichia coli; Food Microbiology; Horses; Life Sciences; Litter; Livestock; Medical Microbiology; Methylene blue; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microorganisms; Mycology; Poultry; Soil contamination; Strains (organisms); Veterinary Microbiology - Research Paper; Wastes
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-023-00912-8

Livestock waste is widely used in agriculture. Although they provide benefits to the soil, and consequently to plants, they have the potential to contaminate the environment, as they contain pathogenic microorganisms and determinants of antimicrobial resistance, if not properly managed. Therefore, this study aims to evaluate the effect of composting horse bedding and poultry litter in organic and conventional production systems on the occurrence of bacteria in the Enterobacteriales order and to identify their antimicrobial resistance profiles. Bacterial strains were isolated from Salmonella-Shigella and eosin methylene blue solid media from animal waste during the composting process that was conducted for 125 days. After isolation, the strains were identified by the MALDI-TOF technique; the disk diffusion test was then performed for phenotypic detection of antimicrobial resistance. A total of 158 bacterial strains were isolated during composting of three wastes. The Enterobacteriaceae family was the most abundant, whereas Proteus mirabilis and Escherichia coli were the species with the highest percentage in the wastes, which also exhibited a multi-resistance profile. Poultry litter showed a greater abundance of resistant bacteria than horse bedding did. Similarly, a greater number of resistant bacteria was detected in conventional poultry litter than in organic poultry litter. The results obtained reinforce that animal wastes are reservoirs of pathogenic bacteria that are resistant to antimicrobials and highlight the importance of developing management strategies that aim to reduce and/or eliminate these contaminants to guarantee their safe use in agriculture.