In silico QTL mapping in an oil palm breeding program reveals a quantitative and complex genetic resistance to Ganoderma boninense

• Published in: Molecular breeding 2021-09, Vol.41 (9), p.53-53, Article 53
• Main Authors: Daval, Aurélie, Pomiès, Virgine, le Squin, Sandrine, Denis, Marie, Riou, Virginie, Breton, Frédéric, Nopariansyah, Bink, Marco, Cochard, Benoît, Jacob, Florence, Billotte, Norbert, Tisné, Sébastien
• Format: Article
• Language: English
• Subjects: Agricultural practices; Agricultural sciences; Biomedical and Life Sciences; Biotechnology; Breeding; Ganoderma; Ganoderma boninense; Gene mapping; Genetic diversity; Genetics; Genotypes; Integrated pest management; Life Sciences; Lignin; Marker-assisted selection; Molecular biology; Nurseries; Palm oil; Pathogens; Pest control; Pest resistance; Plant biology; Plant breeding; Plant diseases; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; Planting; Quantitative trait loci; Stem rot; Vegetable oils; Vegetal Biology
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-021-01246-9

Basal stem rot caused by Ganoderma boninense is the major threat to oil palm cultivation in Southeast Asia, which accounts for 80% of palm oil production worldwide, and this disease is increasing in Africa. The use of resistant planting material as part of an integrated pest management of this disease is one sustainable solution. However, breeding for Ganoderma resistance requires long-term and costly research, which could greatly benefit from marker-assisted selection (MAS). In this study, we evaluated the effectiveness of an in silico genetic mapping approach that took advantage of extensive data recorded in an ongoing breeding program. A pedigree-based QTL mapping approach applied to more than 10 years’ worth of data collected during pre-nursery tests revealed the quantitative nature of Ganoderma resistance and identified underlying loci segregating in genetic diversity that is directly relevant for the breeding program supporting the study. To assess the consistency of QTL effects between pre-nursery and field environments, information was collected on the disease status of the genitors planted in genealogical gardens and modeled with pre-nursery-based QTL genotypes. In the field, individuals were less likely to be infected with Ganoderma when they carried more favorable alleles at the pre-nursery QTL. Our results pave the way for a MAS of Ganoderma resistant and high yielding planting material, and the provided proof-of-concept of this efficient and cost-effective approach could motivate similar studies based on diverse breeding programs.