Efficient identification of palatability-related genes using QTL mapping in rice breeding

• Published in: Molecular breeding 2023-05, Vol.43 (5), p.39-39, Article 39
• Main Authors: Jang, Yoon-Hee, Park, Jae-Ryoung, Kim, Eun-Gyeong, Jan, Rahmatullah, Asif, Saleem, Farooq, Muhammad, Zhao, Dan-Dan, Kim, Kyung-Min
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biosynthesis; Biotechnology; brown rice; Chromosome 6; chromosomes; consumer preferences; doubled haploids; Eating quality; Function analysis; gelatinization temperature; Gene expression; Gene mapping; Genes; Genetic diversity; Genetic resources; genotype; Genotypes; Homology; Life Sciences; Mapping; Molecular biology; Palatability; phenotype; Plant biology; Plant breeding; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; Proteins; Quantitative trait loci; Rice; Starch; Starch synthase; Statistical analysis; Statistical methods; wet digestion method
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-023-01392-2

The gelatinization temperature of rice is an important factor in determining the eating and cooking quality, and it affects consumer preference. The alkali digestion value (ADV) is one of the main methods used to test the quality of rice and has a high correlation with the gelatinization temperature. For the development of high-quality rice, it is important to understand the genetic basis of palatability-related traits, and QTL analysis is a statistical method linking phenotypic data and genotype data and is an effective method to explain the genetic basis of variation in complex traits. QTL mapping related to the ADV of brown and milled rice was performed using the 120 Cheongcheong/Nagdong double haploid (CNDH) line. As a result, 12 QTLs related to ADV were detected, and 20 candidate genes were selected from the RM588–RM1163 region of chromosome 6 through screening by gene function analysis. The comparison of the relative expression level of candidate genes showed that OsSS1q6 is highly expressed in CNDH lines with high ADV in both brown rice and milled rice. In addition, OsSS1q6 has high homology with the starch synthase 1 protein and interacts with various starch biosynthesis-related proteins, such as GBSSII, SBE, and APL. Therefore, we suggest that OsSS1q6 identified through QTL mapping could be one of the various genes involved in the gelatinization temperature of rice by regulating starch biosynthesis. This study can be used as basic data for breeding high-quality rice and provides a new genetic resource that can increase the palatability of rice.