Loading…

The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite

The genetically related assemblages of the intestinal protozoa parasite are morphologically indistinguishable and are often derived from specific hosts. The assemblages are separated by large genetic distances, which might account for their relevant biological and pathogenic differences. In this wor...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2023-05, Vol.24 (11), p.9559
Main Authors: Natali, Lautaro, Luna Pizarro, Gabriel, Moyano, Sofía, de la Cruz-Thea, Benjamin, Musso, Juliana, Rópolo, Andrea S, Eichner, Norbert, Meister, Gunter, Musri, Melina M, Feliziani, Constanza, Touz, María C
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The genetically related assemblages of the intestinal protozoa parasite are morphologically indistinguishable and are often derived from specific hosts. The assemblages are separated by large genetic distances, which might account for their relevant biological and pathogenic differences. In this work, we analyzed the RNAs cargo released into exosomal-like vesicles (ElVs) by the assemblages A and B, which differentially infect humans, and the assemblage E, which infects hoofed animals. The RNA sequencing analysis revealed that the ElVs of each assemblage contained distinct small RNA (sRNA) biotypes, suggesting a preference for specific packaging in each assemblage. These sRNAs were classified into three categories, ribosomal-small RNAs (rsRNAs), messenger-small RNAs (msRNAs), and transfer-small RNAs (tsRNAs), which may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis. Uptake experiments showed, for the first time, that ElVs were successfully internalized by the parasite trophozoites. Furthermore, we observed that the sRNAs contained inside these ElVs were first located below the plasma membrane but then distributed along the cytoplasm. Overall, the study provides new insights into the molecular mechanisms underlying the host-specificity and pathogenesis of and highlights the potential role of sRNAs in parasite communication and regulation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24119559