Hyponastic Leaves 1 Interacts with RNA Pol II to Ensure Proper Transcription of MicroRNA Genes

• Published in: Plant and cell physiology 2023-06, Vol.64 (6), p.571-582
• Main Authors: Bielewicz, Dawid, Dolata, Jakub, Bajczyk, Mateusz, Szewc, Lukasz, Gulanicz, Tomasz, Bhat, Susheel S, Karlik, Anna, Jozwiak, Monika, Jarmolowski, Artur, Szweykowska-Kulinska, Zofia
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Editor's Choice; Gene Expression Regulation, Plant; MicroRNAs - genetics; MicroRNAs - metabolism; Plant Leaves - genetics; Plant Leaves - metabolism; Proteomics; Regular Paper; RNA Polymerase II - genetics; RNA Polymerase II - metabolism; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcad032

Abstract Hyponastic Leaves 1 (HYL1) [also known as Double-stranded RNA-Binding protein 1 (DRB1)] is a double-stranded RNA-binding protein involved in microRNA (miRNA) processing in plants. It is a core component of the Microprocessor complex and enhances the efficiency and precision of miRNA processing by the Dicer-Like 1 protein. In this work, we report a novel function of the HYL1 protein in the transcription of miRNA (MIR) genes. HYL1 colocalizes with RNA polymerase II and affects its distribution along MIR genes. Moreover, proteomic experiments revealed that the HYL1 protein interacts with many transcription factors. Finally, we show that the action of HYL1 is not limited to MIR genes and impacts the expression of many other genes, a majority of which are involved in plastid organization. These discoveries indicate HYL1 as an additional player in gene regulation at the transcriptional level, independent of its role in miRNA biogenesis.