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A unique ISRE, in the TATA-less human Isg20 promoter, confers IRF-1-mediated responsiveness to both interferon type I and type II
Interferons (IFNs) encode a family of secreted proteins involved in a number of regulatory functions such as control of cell proliferation, differentiation and regulation of the immune system. Their diverse biological actions are thought to be mediated by the products of specific but usually overlap...
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Published in: | Nucleic acids research 2000-06, Vol.28 (12), p.2333-2341 |
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description | Interferons (IFNs) encode a family of secreted proteins involved in a number of regulatory functions such as control of cell proliferation, differentiation and regulation of the immune system. Their diverse biological actions are thought to be mediated by the products of specific but usually overlapping sets of cellular genes induced in the target cells. We have recently isolated a human cDNA encoding a new nuclear bodies-associated protein (PML-NBs), which we have termed Isg20. In this report, we describe the cloning and functional characterization of the Isg20 promoter region and the identification of sequence elements and trans-acting factors implicated in its regulation. In the absence of any recognizable TATA or CAAT elements, Isg20 promoter basal activity is dependent upon the positive transcription factors Sp-1 and USF-1. Interestingly, we demonstrate that a unique interferon stimulated response element (ISRE) mediates both IFN type I and type II Isg20 induction in the absence of functional gamma-activated sequence. These inductions are strictly dependent upon of the IFN regulatory factor 1 (IRF-1). In addition, we show that the ISRE is also implicated in the constitutive transcriptional activity of Isg20 gene. |
doi_str_mv | 10.1093/nar/28.12.2333 |
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Their diverse biological actions are thought to be mediated by the products of specific but usually overlapping sets of cellular genes induced in the target cells. We have recently isolated a human cDNA encoding a new nuclear bodies-associated protein (PML-NBs), which we have termed Isg20. In this report, we describe the cloning and functional characterization of the Isg20 promoter region and the identification of sequence elements and trans-acting factors implicated in its regulation. In the absence of any recognizable TATA or CAAT elements, Isg20 promoter basal activity is dependent upon the positive transcription factors Sp-1 and USF-1. Interestingly, we demonstrate that a unique interferon stimulated response element (ISRE) mediates both IFN type I and type II Isg20 induction in the absence of functional gamma-activated sequence. These inductions are strictly dependent upon of the IFN regulatory factor 1 (IRF-1). 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Their diverse biological actions are thought to be mediated by the products of specific but usually overlapping sets of cellular genes induced in the target cells. We have recently isolated a human cDNA encoding a new nuclear bodies-associated protein (PML-NBs), which we have termed Isg20. In this report, we describe the cloning and functional characterization of the Isg20 promoter region and the identification of sequence elements and trans-acting factors implicated in its regulation. In the absence of any recognizable TATA or CAAT elements, Isg20 promoter basal activity is dependent upon the positive transcription factors Sp-1 and USF-1. Interestingly, we demonstrate that a unique interferon stimulated response element (ISRE) mediates both IFN type I and type II Isg20 induction in the absence of functional gamma-activated sequence. These inductions are strictly dependent upon of the IFN regulatory factor 1 (IRF-1). In addition, we show that the ISRE is also implicated in the constitutive transcriptional activity of Isg20 gene.</description><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biochemistry, Molecular Biology</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>DNA-Binding Proteins</subject><subject>Exonucleases</subject><subject>Exoribonucleases</subject><subject>Gene Expression Regulation</subject><subject>Genomic Library</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>interferon regulatory factor 1</subject><subject>Isg20 gene</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sp1 Transcription Factor - metabolism</subject><subject>TATA Box</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>Upstream Stimulatory Factors</subject><issn>1362-4962</issn><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpdkkFr3DAQhUVpaNK01x6L6KFQiDcayZbtQw8mJI1hoZBuz0KS5djBllzJXsix_7wyu5RNTho035s3Gg1Cn4BsgJTs2kp_TYsN0A1ljL1BF8A4TdKS07cn8Tl6H8ITIZBClr5D50CKPCazC_S3wovt_ywG178ebq9wb_HcGbyrdlUymBBwt4zS4jo8UoIn70Y3G3-FtbOt8QHXD3cJJKNpejmbBnsTJmdDvzd21c4OKzd3sWgURd7F4s9TtMLSNsew_oDOWjkE8_F4XqLfd7e7m_tk-_NHfVNtE52Sck5YCy3PIVOMpUSpLNc5U6wtmGzbsjBQNk1BCq2VVmlDWUNLw7NcySZtNJRFxi7R90PdaVGxYW3s7OUgJt-P0j8LJ3vxMmP7Tjy6vQBCc7bqvx303SvVfbUV6x2hjAOBcg-R_Xr08i7ONsxi7IM2wyCtcUsQkGcZ55xF8Msr8Mkt3sY5CEoIZ4SQPEKbA6S9C8Gb9r89ELFugYhbIGghgIp1C6Lg8-lTT_DDt7N_hj-tPg</recordid><startdate>20000615</startdate><enddate>20000615</enddate><creator>Gongora, C</creator><creator>Degols, G</creator><creator>Espert, L</creator><creator>Hua, T D</creator><creator>Mechti, N</creator><general>Oxford Publishing Limited (England)</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9034-4031</orcidid></search><sort><creationdate>20000615</creationdate><title>A unique ISRE, in the TATA-less human Isg20 promoter, confers IRF-1-mediated responsiveness to both interferon type I and type II</title><author>Gongora, C ; Degols, G ; Espert, L ; Hua, T D ; Mechti, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-3f1f6715b3340bb57c73b3f83aff98e19dd808ccbcb4d23d29e657bad4dc19853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biochemistry, Molecular Biology</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cloning, Molecular</topic><topic>Conserved Sequence</topic><topic>DNA-Binding Proteins</topic><topic>Exonucleases</topic><topic>Exoribonucleases</topic><topic>Gene Expression Regulation</topic><topic>Genomic Library</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>interferon regulatory factor 1</topic><topic>Isg20 gene</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sp1 Transcription Factor - metabolism</topic><topic>TATA Box</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>Upstream Stimulatory Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gongora, C</creatorcontrib><creatorcontrib>Degols, G</creatorcontrib><creatorcontrib>Espert, L</creatorcontrib><creatorcontrib>Hua, T D</creatorcontrib><creatorcontrib>Mechti, N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gongora, C</au><au>Degols, G</au><au>Espert, L</au><au>Hua, T D</au><au>Mechti, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A unique ISRE, in the TATA-less human Isg20 promoter, confers IRF-1-mediated responsiveness to both interferon type I and type II</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2000-06-15</date><risdate>2000</risdate><volume>28</volume><issue>12</issue><spage>2333</spage><epage>2341</epage><pages>2333-2341</pages><issn>1362-4962</issn><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Interferons (IFNs) encode a family of secreted proteins involved in a number of regulatory functions such as control of cell proliferation, differentiation and regulation of the immune system. Their diverse biological actions are thought to be mediated by the products of specific but usually overlapping sets of cellular genes induced in the target cells. We have recently isolated a human cDNA encoding a new nuclear bodies-associated protein (PML-NBs), which we have termed Isg20. In this report, we describe the cloning and functional characterization of the Isg20 promoter region and the identification of sequence elements and trans-acting factors implicated in its regulation. In the absence of any recognizable TATA or CAAT elements, Isg20 promoter basal activity is dependent upon the positive transcription factors Sp-1 and USF-1. Interestingly, we demonstrate that a unique interferon stimulated response element (ISRE) mediates both IFN type I and type II Isg20 induction in the absence of functional gamma-activated sequence. These inductions are strictly dependent upon of the IFN regulatory factor 1 (IRF-1). 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subjects | Base Sequence Binding Sites Biochemistry, Molecular Biology Carrier Proteins - genetics Carrier Proteins - metabolism Cell Nucleus - metabolism Cloning, Molecular Conserved Sequence DNA-Binding Proteins Exonucleases Exoribonucleases Gene Expression Regulation Genomic Library HeLa Cells Humans interferon regulatory factor 1 Isg20 gene Life Sciences Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins - genetics Nuclear Proteins - metabolism Promoter Regions, Genetic Recombinant Proteins - metabolism Sp1 Transcription Factor - metabolism TATA Box Transcription Factors - metabolism Transcription, Genetic Transfection Tumor Cells, Cultured Upstream Stimulatory Factors |
title | A unique ISRE, in the TATA-less human Isg20 promoter, confers IRF-1-mediated responsiveness to both interferon type I and type II |
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