Multimodal image reconstruction from tomographic diffraction microscopy data

Summary Tomographic diffraction microscopy (TDM) is a tool of choice for high‐resolution, marker‐less 3D imaging of biological samples. Based on a generalization of digital holographic microscopy with full control of the sample's illumination, TDM measures, from many illumination directions, th...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2022-12, Vol.288 (3), p.193-206
Main Authors: Abbessi, Riadh, Verrier, Nicolas, Taddese, Asemare Mengistie, Laroche, Steve, Debailleul, Matthieu, Lo, Mohamed, Courbot, Jean‐Baptiste, Haeberlé, Olivier
Format: Article
Language:English
Subjects:
Biological properties
Diffraction
Digital imaging
Engineering Sciences
holography
Holography - methods
Illumination
Image processing
Image Processing, Computer-Assisted - methods
Image reconstruction
Microscopy
Microscopy - methods
Microscopy, Phase-Contrast
Optics
Photonic
Phototoxicity
Refractivity
Themed Issue
Tomography
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Summary:Summary Tomographic diffraction microscopy (TDM) is a tool of choice for high‐resolution, marker‐less 3D imaging of biological samples. Based on a generalization of digital holographic microscopy with full control of the sample's illumination, TDM measures, from many illumination directions, the diffracted fields in both phase and amplitude. Photon budget associated to TDM imaging is low. Therefore, TDM is not limited by phototoxicity issues. The recorded information makes it possible to reconstruct 3D refractive index distribution (with both refraction and absorption contributions) of the object under scrutiny, without any staining. In this contribution, we show an alternate use of this information. A tutorial for multimodal image reconstruction is proposed. Both intensity contrasts and phase contrasts are proposed, from the image formation model to the final reconstruction with both 2D and 3D rendering, turning TDM into a kind of ‘universal’ digital microscope.
ISSN:0022-2720
1365-2818
DOI:10.1111/jmi.13131