Probabilistic establishment of speckle-associated inter-chromosomal interactions

Abstract Inter-chromosomal interactions play a crucial role in genome organization, yet the organizational principles remain elusive. Here, we introduce a novel computational method to systematically characterize inter-chromosomal interactions using in situ Hi-C results from various cell types. Our...

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Bibliographic Details
Published in:Nucleic acids research 2023-06, Vol.51 (11), p.5377-5395
Main Authors: Joo, Jaegeon, Cho, Sunghyun, Hong, Sukbum, Min, Sunwoo, Kim, Kyukwang, Kumar, Rajeev, Choi, Jeong-Mo, Shin, Yongdae, Jung, Inkyung
Format: Article
Language:English
Subjects:
Cell Nucleus - metabolism
Chromatin - genetics
Chromatin - metabolism
Chromosomes
DNA - genetics
DNA - metabolism
Gene regulation, Chromatin and Epigenetics
Humans
In Situ Hybridization, Fluorescence
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Summary:Abstract Inter-chromosomal interactions play a crucial role in genome organization, yet the organizational principles remain elusive. Here, we introduce a novel computational method to systematically characterize inter-chromosomal interactions using in situ Hi-C results from various cell types. Our method successfully identifies two apparently hub-like inter-chromosomal contacts associated with nuclear speckles and nucleoli, respectively. Interestingly, we discover that nuclear speckle-associated inter-chromosomal interactions are highly cell-type invariant with a marked enrichment of cell-type common super-enhancers (CSEs). Validation using DNA Oligopaint fluorescence in situ hybridization (FISH) shows a strong but probabilistic interaction behavior between nuclear speckles and CSE-harboring genomic regions. Strikingly, we find that the likelihood of speckle-CSE associations can accurately predict two experimentally measured inter-chromosomal contacts from Hi-C and Oligopaint DNA FISH. Our probabilistic establishment model well describes the hub-like structure observed at the population level as a cumulative effect of summing individual stochastic chromatin-speckle interactions. Lastly, we observe that CSEs are highly co-occupied by MAZ binding and MAZ depletion leads to significant disorganization of speckle-associated inter-chromosomal contacts. Taken together, our results propose a simple organizational principle of inter-chromosomal interactions mediated by MAZ-occupied CSEs.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkad211