Metagenomic analysis of ready biodegradability tests to ascertain the relationship between microbiota and the biodegradability of test chemicals

Ready biodegradability tests conducted in accordance with the Organisation for Economic Co-operation and Development guidelines (test 301C or test 301F) are performed using activated sludge (AS) prepared by the Chemicals Evaluation and Research Institute (AS-CERI) or that taken from a sewage treatme...

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Bibliographic Details
Published in:Journal of Pesticide Science 2023/05/20, Vol.48(2), pp.35-46
Main Authors: Takano, Yoshinari, Takekoshi, Saki, Takano, Kotaro, Matoba, Yoshihide, Mukumoto, Makiko, Shirai, Osamu
Format: Article
Language:English
Subjects:
Activated sludge
Biodegradability
Biodegradation
Chemicals
Community structure
degrader
metagenomic analysis
Metagenomics
Microbiota
Microorganisms
ready biodegradability test
Regular
Sewage treatment plants
test medium volume
Wastewater treatment plants
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Summary:Ready biodegradability tests conducted in accordance with the Organisation for Economic Co-operation and Development guidelines (test 301C or test 301F) are performed using activated sludge (AS) prepared by the Chemicals Evaluation and Research Institute (AS-CERI) or that taken from a sewage treatment plant (AS-STP). It had been reported that AS-CERI had lower activity than AS-STP in biodegrading test chemicals, and that biodegradation was accelerated by increasing the volume of the test medium. However, these phenomena have not been clarified from the perspective of the microbiota. In this study, using metagenomic analysis, we first showed that the microbiota of AS-CERI was biased in its distribution of phyla, less diverse, and had greater lot-to-lot variability than that of AS-STP. Second, after cultivation for a long period of time, the microbiota of AS-STP and AS-CERI became more similar to each other in terms of community structure. Third, determining degraders of test substances when each substance was actively biodegraded was found to be an effective approach. Finally, we clarified experimentally that a large volume of test medium increased the number of species that could degrade test substances in the condition where the initial concentrations of each substance and AS-STP were kept constant.
ISSN:1348-589X
1349-0923
DOI:10.1584/jpestics.D22-067