Genomes comparison of two Proteus mirabilis clones showing varied swarming ability

Background Proteus mirabilis is a Gram-negative bacteria most noted for its involvement with catheter-associated urinary tract infections. It is also known for its multicellular migration over solid surfaces, referred to as ‘swarming motility’. Here we analyzed the genomic sequences of two P. mirabi...

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Bibliographic Details
Published in:Molecular biology reports 2023-07, Vol.50 (7), p.5817-5826
Main Authors: Gmiter, Dawid, Pacak, Ilona, Nawrot, Sylwia, Czerwonka, Grzegorz, Kaca, Wieslaw
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Catheters
Clone Cells
Genomes
Genomics
Gram-negative bacteria
Histology
Host-pathogen interactions
Humans
Life Sciences
Morphology
Motility
Original
Original Article
Proteus Infections - microbiology
Proteus mirabilis
Proteus mirabilis - genetics
Swarming
Urinary tract
Urinary tract diseases
Urinary tract infections
Urinary Tract Infections - genetics
Urinary Tract Infections - microbiology
Urogenital system
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Summary:Background Proteus mirabilis is a Gram-negative bacteria most noted for its involvement with catheter-associated urinary tract infections. It is also known for its multicellular migration over solid surfaces, referred to as ‘swarming motility’. Here we analyzed the genomic sequences of two P. mirabilis isolates, designated K38 and K39, which exhibit varied swarming ability. Methods and results The isolates genomes were sequenced using Illumina NextSeq sequencer, resulting in about 3.94 Mbp, with a GC content of 38.6%, genomes. Genomes were subjected for in silico comparative investigation. We revealed that, despite a difference in swarming motility, the isolates showed high genomic relatedness (up to 100% ANI similarity), suggesting that one of the isolates probably originated from the other. Conclusions The genomic sequences will allow us to investigate the mechanism driving this intriguing phenotypic heterogeneity between closely related P. mirabilis isolates. Phenotypic heterogeneity is an adaptive strategy of bacterial cells to several environmental pressures. It is also an important factor related to their pathogenesis. Therefore, the availability of these genomic sequences will facilitate studies that focus on the host–pathogen interactions during catheter-associated urinary tract infections.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-023-08518-x