Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant Sublineages

The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer...

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Bibliographic Details
Published in:Genes 2023-06, Vol.14 (6), p.1218
Main Authors: Esman, Anna, Dubodelov, Dmitry, Khafizov, Kamil, Kotov, Ivan, Roev, German, Golubeva, Anna, Gasanov, Gasan, Korabelnikova, Marina, Turashev, Askar, Cherkashin, Evgeniy, Mironov, Konstantin, Cherkashina, Anna, Akimkin, Vasily
Format: Article
Language:English
Subjects:
COVID-19 - diagnosis
COVID-19 - genetics
COVID-19 Testing
Databases, Factual
Epidemiology
Genomes
Humans
Immunization
Mutation
Phylogenetics
Proteins
Reagents
Real-Time Polymerase Chain Reaction
SARS-CoV-2 - genetics
Severe acute respiratory syndrome coronavirus 2
Software
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Summary:The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer panels for RT-PCR and Sanger and NGS sequencing methods. For the centralized collection and analysis of samples, the VGARus database was developed, which currently includes more than 300,000 viral sequences.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes14061218