Quorum Sensing Is Required for the Colony Establishment of a Plant Phyllosphere Bacterium Rhodopseudomonas palustris Strain GJ-22

• Published in: Applied and environmental microbiology 2023-06, Vol.89 (6), p.e0048723-e0048723
• Main Authors: Zhang, Weixing, Peng, Qianze, Chen, Lijie, Gu, Zepei, Liu, Zhuoxin, Zhang, Deyong, Cheng, Ju'e, Zheng, Limin, Chen, Ang, Liu, Yong, Su, Pin
• Format: Article
• Language: English
• Subjects: Acyl-Butyrolactones; Aggregates; Bacteria; Biological control; Biosynthesis; Cell aggregation; Environmental Microbiology; Exopolysaccharides; Hydrogen Peroxide; Intermediates; Phyllosphere; Plant Microbiology; Quorum sensing; Quorum Sensing - genetics; Reactive oxygen species; Rhizosphere; Rhodopseudomonas - genetics; Rhodopseudomonas palustris; Signal Transduction
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.00487-23

The phyllosphere presents a hostile environment for many biocontrol agents; however, it is as significant as is the rhizosphere for plant health. Deploying biocontrol bacteria into the phyllosphere can efficiently suppress diseases; however, the lack of knowledge on the phyllosphere adaptive traits of biocontrol bacteria poses challenges. In this study, we demonstrated that Rhodopseudomonas palustris GJ-22 colonizes the phyllosphere by forming cell aggregates. The formation of cell aggregates required the production of exopolysaccharides (EPS), which depended on the function of the quorum sensing (QS) mechanism, mediated by the signaling molecule -coumaroyl-HSL ( C-HSL). The mutation of the EPS biosynthesis gene or the signaling molecule biosynthesis gene compromised the ability of GJ-22 to tolerate reactive oxygen intermediates (ROIs), such as H O , and to form cell aggregates . Collectively, the results revealed that QS mediates EPS production and consequently leads to bacterial cell aggregation. Quorum sensing is used by various bacteria for coordinating the multiplication of bacterial cells in a group and for modulating the behaviors of surrounding microbial species. Host plants can benefit from this interspecies modulation, as it can disrupt the QS circuits of pathogenic bacteria. Some N-acyl homoserine lactone- (AHL-) producing bacteria that were introduced into the phyllosphere as biocontrol agents may establish AHL-based crosstalk with indigenous microbes to steer the nutritional and microecological conditions toward their own and the host plant's benefit. Here, we showed that biocontrol bacteria introduced into the phyllosphere require a functioning QS circuit to establish colonies and suppress pathogens. Furthermore, our findings provoked a broader investigation into the role of the QS circuit in beneficial microorganism-plant interactions.