The Caenorhabditis elegans innexin INX-20 regulates nociceptive behavioral sensitivity

• Published in: Genetics (Austin) 2023-04, Vol.223 (4)
• Main Authors: Chaubey, Aditi H, Sojka, Savannah E, Onukwufor, John O, Ezak, Meredith J, Vandermeulen, Matthew D, Bowitch, Alexander, Vodičková, Anežka, Wojtovich, Andrew P, Ferkey, Denise M
• Format: Article
• Language: English
• Subjects: Ablation; Animals; Ashes; Caenorhabditis elegans; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Chemical stimuli; CRISPR; Gap Junctions; Hypersensitivity; Investigation; Locomotion; Nematodes; Neural networks; Neurons; Nociception; Nociceptors; Pain perception; Predators; Sensory neurons; Sensory Receptor Cells - metabolism; Stimuli
• ISSN: 1943-2631; 0016-6731; 1943-2631
• DOI: 10.1093/genetics/iyad017

Abstract Organisms rely on chemical cues in their environment to indicate the presence or absence of food, reproductive partners, predators, or other harmful stimuli. In the nematode Caenorhabditis elegans, the bilaterally symmetric pair of ASH sensory neurons serves as the primary nociceptors. ASH activation by aversive stimuli leads to backward locomotion and stimulus avoidance. We previously reported a role for guanylyl cyclases in dampening nociceptive sensitivity that requires an innexin-based gap junction network to pass cGMP between neurons. Here, we report that animals lacking function of the gap junction component INX-20 are hypersensitive in their behavioral response to both soluble and volatile chemical stimuli that signal through G protein-coupled receptor pathways in ASH. We find that expressing inx-20 in the ADL and AFD sensory neurons is sufficient to dampen ASH sensitivity, which is supported by new expression analysis of endogenous INX-20 tagged with mCherry via the CRISPR-Cas9 system. Although ADL does not form gap junctions directly with ASH, it does so via gap junctions with the interneuron RMG and the sensory neuron ASK. Ablating either ADL or RMG and ASK also resulted in nociceptive hypersensitivity, suggesting an important role for RMG/ASK downstream of ADL in the ASH modulatory circuit. This work adds to our growing understanding of the repertoire of ways by which ASH activity is regulated via its connectivity to other neurons and identifies a previously unknown role for ADL and RMG in the modulation of aversive behavior.