Development of surface functionalization strategies for 3D‐printed polystyrene constructs

There is a growing interest in 3D printing to fabricate culture substrates; however, the surface properties of the scaffold remain pertinent to elicit targeted and expected cell responses. Traditional 2D polystyrene (PS) culture systems typically require surface functionalization (oxidation) to faci...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2019-11, Vol.107 (8), p.2566-2578
Main Authors: Lerman, Max J., Muramoto, Shin, Arumugasaamy, Navein, Van Order, Michael, Lembong, Josephine, Gerald, Anushka G., Gillen, Greg, Fisher, John P.
Format: Article
Language:English
Subjects:
3-D printers
3D printing
Adhesion
Animals
Biomedical materials
Carbonyl compounds
Carbonyl groups
Carbonyls
Cattle
Cell adhesion
Cell adhesion & migration
Cell culture
Cellular manufacture
Contact angle
Culture
Extracellular matrix
Humans
Materials research
Materials science
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mesenchyme
Oxidation
plasma treatment
Polystyrene
Polystyrene resins
Polystyrenes - chemistry
Printing, Three-Dimensional
protein adhesion
Proteins
Scaffolds
Stem cells
Substrates
surface chemical modification
Surface Properties
Three dimensional printing
Tissue Scaffolds - chemistry
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Summary:There is a growing interest in 3D printing to fabricate culture substrates; however, the surface properties of the scaffold remain pertinent to elicit targeted and expected cell responses. Traditional 2D polystyrene (PS) culture systems typically require surface functionalization (oxidation) to facilitate and encourage cell adhesion. Determining the surface properties which enhance protein adhesion from media and cellular extracellular matrix (ECM) production remains the first step to translating 2D PS systems to a 3D culture surface. Here we show that the presence of carbonyl groups to PS surfaces correlated well with successful adhesion of ECM proteins and sustaining ECM production of deposited human mesenchymal stem cells, if the surface has a water contact angle between 50° and 55°. Translation of these findings to custom‐fabricated 3D PS scaffolds reveals carbonyl groups continued to enhance spreading and growth in 3D culture. Cumulatively, these data present a method for 3D printing PS and the design considerations required for understanding cell–material interactions. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2566–2578, 2019.
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.34347