Investigating ionizing radiation-induced changes in breast cancer cells using stimulated Raman scattering microscopy

• Published in: Journal of biomedical optics 2023-07, Vol.28 (7), p.076501-076501
• Main Authors: Allen, Christian Harry, Skillings, Robyn, Ahmed, Duale, Sanchez, Sarita Cuadros, Altwasser, Kaitlyn, Hilan, George, Willmore, William G, Chauhan, Vinita, Cassol, Edana, Murugkar, Sangeeta
• Format: Article
• Language: English
• Subjects: Apoptosis; Breast cancer; Breast Neoplasms - diagnostic imaging; Cancer therapies; Cell culture; Cell death; Cell size; Cell viability; Cytology; Female; Flow cytometry; Fluorescence; Humans; Imaging; Ionizing radiation; Irradiation; Labels; Lasers; Lipid metabolism; Lipid peroxidation; Lipids; Metabolism; Microscopy; Morphology; Nonlinear Optical Microscopy; Peroxidation; Pilot Projects; Proteins; Radiation; Radiation effects; Radiation tolerance; Radiation, Ionizing; Raman spectra; Spectrum analysis; Spectrum Analysis, Raman - methods; Toxicity; X-rays
• ISSN: 1083-3668; 1560-2281
• DOI: 10.1117/1.JBO.28.7.076501

Altered lipid metabolism of cancer cells has been implicated in increased radiation resistance. A better understanding of this phenomenon may lead to improved radiation treatment planning. Stimulated Raman scattering (SRS) microscopy enables label-free and quantitative imaging of cellular lipids but has never been applied in this domain. We sought to investigate the radiobiological response in human breast cancer MCF7 cells using SRS microscopy, focusing on how radiation affects lipid droplet (LD) distribution and cellular morphology. MCF7 breast cancer cells were exposed to either 0 or 30 Gy (X-ray) ionizing radiation and imaged using a spectrally focused SRS microscope every 24 hrs over a 72-hr time period. Images were analyzed to quantify changes in LD area per cell, lipid and protein content per cell, and cellular morphology. Cell viability and confluency were measured using a live cell imaging system while radiation-induced lipid peroxidation was assessed using BODIPY C11 staining and flow cytometry. The LD area per cell and total lipid and protein intensities per cell were found to increase significantly for irradiated cells compared to control cells from 48 to 72 hrs post irradiation. Increased cell size, vacuole formation, and multinucleation were observed as well. No significant cell death was observed due to irradiation, but lipid peroxidation was found to be greater in the irradiated cells than control cells at 72 hrs. This pilot study demonstrates the potential of SRS imaging for investigating ionizing radiation-induced changes in cancer cells without the use of fluorescent labels.