Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

Nudt2 encodes a diadenosine tetraphosphate (Ap A) hydrolase that catalyzes the hydrolysis of Ap A and is involved in the lysyl tRNA synthetase-Ap A-Nudt2 (LysRS-Ap A-Nudt2) signaling pathway. We have previously demonstrated that this pathway is active in non-small cell lung cancer. Nudt2 was shown t...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-06, Vol.24 (13), p.10513
Main Authors: Hidmi, Sana', Nechushtan, Hovav, Razin, Ehud, Tshori, Sagi
Format: Article
Language:English
Subjects:
Anchorages
Animals
Breast cancer
Cancer
Cancer therapies
Carcinoma, Non-Small-Cell Lung
Care and treatment
Cell adhesion & migration
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Cell proliferation
Cell Proliferation - genetics
Epithelial-Mesenchymal Transition - genetics
Estrogens
Ethylenediaminetetraacetic acid
Growth
Health aspects
Humans
In vivo methods and tests
Leukemia
Lung cancer
Lung cancer, Non-small cell
Lung Neoplasms
Melanoma
Melanoma - metabolism
Metastasis
Mice
Non-small cell lung carcinoma
Protein expression
Signal transduction
Skin cancer
Stem cells
tRNA
Tumorigenesis
Tumors
Vimentin
Xenotransplantation
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Summary:Nudt2 encodes a diadenosine tetraphosphate (Ap A) hydrolase that catalyzes the hydrolysis of Ap A and is involved in the lysyl tRNA synthetase-Ap A-Nudt2 (LysRS-Ap A-Nudt2) signaling pathway. We have previously demonstrated that this pathway is active in non-small cell lung cancer. Nudt2 was shown to be involved in cell proliferation in breast cancer, making it an important target in cancer therapy. Currently, the function of Nudt2 in malignant melanoma has not been demonstrated. Therefore, we investigated the role played by Nudt2 in the growth of human melanoma. Our study showed that Nudt2 knockdown suppressed anchorage-independent growth of human melanoma cells in vitro. The in vivo effect of Nudt2 was determined by investigating the role played by Nudt2 knockdown on the ability of the cells to form tumors in a mice xenograft model. Nudt2 knockdown significantly suppressed tumor growth in this model. Moreover, overexpression of Nudt2 resulted in an increase in anchorage-independent growth of these cells, whereas Nudt2 knockdown decreased their migration. In addition, Nudt2 knockdown reduced vimentin expression. Vimentin is one of the mesenchymal markers that are involved in the epithelial mesenchymal transition (EMT) process. Thus, Nudt2 plays an important role in promoting anchorage-independent growth and cell migration in melanoma.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241310513