Overexpression of TaMYB4 Confers Freezing Tolerance in Arabidopsis thaliana

Freezing stress is one of the main factors limiting the growth and yield of wheat. In this study, we found that expression was significantly upregulated in the tillering nodes of the strong cold-resistant winter wheat variety Dongnongdongmai1 (Dn1) under freezing stress. Weighted gene co-expression...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-07, Vol.24 (13), p.11090
Main Authors: Tian, Yu, Peng, Kankan, Ma, Xuan, Ren, Zhipeng, Lou, Guicheng, Jiang, Yunshuang, Xia, Jingqiu, Wang, Duojia, Yu, Jing, Cang, Jing
Format: Article
Language:English
Subjects:
Abiotic stress
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Ascorbic acid
Binding sites
CBF protein
Cloning
Cold
Cold tolerance
Cold-Shock Response - genetics
Crop yield
Cytoplasm
Freezing
Gene expression
Gene Expression Regulation, Plant
Leaves
Localization
Low temperature resistance
Network analysis
Phylogenetics
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Proteins
Reductases
Signal transduction
Temperature
Tobacco
Transcription Factors - genetics
Transcription Factors - metabolism
Wheat
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Summary:Freezing stress is one of the main factors limiting the growth and yield of wheat. In this study, we found that expression was significantly upregulated in the tillering nodes of the strong cold-resistant winter wheat variety Dongnongdongmai1 (Dn1) under freezing stress. Weighted gene co-expression network analysis, qRT-PCR and protein-DNA interaction experiments demonstrated that monodehydroascorbate reductase ( ) is a direct target of TaMYB4. The results showed that overexpression of enhanced the freezing tolerance of transgenic . In overexpression lines (OE- ), expression was upregulated and ascorbate-glutathione (AsA-GSH) cycle operation was enhanced. In addition, the expression of cold stress marker genes such as , , , , , and in OE- lines was significantly upregulated. Therefore, TaMYB4 may increase freezing tolerance as a transcription factor (TF) in through the AsA-GSH cycle and DREB/CBF signaling pathway. This study provides a potential gene for molecular breeding against freezing stress.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241311090