Spent coffee grounds as feedstock for the production of biosurfactants and the improved recovery of melanoidins

Spent coffee grounds (SCG) are wastes generated in high amounts worldwide. Their composition makes them a promising feedstock for biotechnological processes. Here we show that the production of the biosurfactant surfactin by submerged culture of a Bacillus subtilis strain growing on SCG is possible,...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2023-09, Vol.39 (9), p.254-254, Article 254
Main Authors: Moya-Ramírez, Ignacio, Pegalajar-Robles, María Encarnación, Debiasi Alberton, Michele, Rufián-Henares, José A., Fernández-Arteaga, Alejandro, Garcia-Roman, Miguel, Altmajer-Vaz, Deisi
Format: Article
Language:English
Subjects:
Antioxidants
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Bioremediation
Biosurfactants
Biotechnology
Cell culture
Coffee
Congeners
Cosmetics
Environmental Engineering/Biotechnology
Life Sciences
Light scattering
Melanoidin
Melanoidins
Microbiology
Photon correlation spectroscopy
Polymers
Raw materials
Recovery
Soil amendment
Strong interactions (field theory)
Surfactants
Surfactin
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Summary:Spent coffee grounds (SCG) are wastes generated in high amounts worldwide. Their composition makes them a promising feedstock for biotechnological processes. Here we show that the production of the biosurfactant surfactin by submerged culture of a Bacillus subtilis strain growing on SCG is possible, reaching concentrations up to 8.8 mg/L when using SCG at 8.3 g/L in the medium. In addition, we report a synergy between the production of surfactin and the recovery of melanoidins, an added-value compound already present in SCG. More specifically, the concentration of melanoidins in the culture medium increased between 2.1 and 2.5 times thanks to the presence of the B. subtilis in the culture. Furthermore, we have observed a strong interaction between surfactin and melanoidin aggregates through dynamic light scattering measurements, and that both of them can be co-purified with an acid precipitation. We have also characterized the interfacial and antioxidant properties of the cell-free supernatant and surfactin extract, as well as the distribution of the congeners of the biosurfactant. Altogether, this work describes a promising approach to obtain biosurfactants and antioxidant molecules in a single operation, which can be used to design several new formulations of interest for bioremediation, amendment of soils, food and cosmetics.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-023-03698-x