Determination of urinary 2,5-hexanedione concentration by an improved analytical method as an index of exposure to n-hexane

2,5-Hexanedione is a main metabolite of n-hexane and is considered as the cause of n-hexane polyneuropathy. Therefore, it is useful to measure 2,5-hexanedione for biological monitoring of exposure to n-hexane. The analytical methods existing for n-hexane metabolites, however, were controversial and...

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Bibliographic Details
Published in:British Journal of Industrial Medicine 1991-08, Vol.48 (8), p.568-574
Main Authors: Saito, I, Shibata, E, Huang, J, Hisanaga, N, Ono, Y, Takeuchi, Y
Format: Article
Language:English
Subjects:
Acid hydrolysis
Adhesives
Animals
Automobiles
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Chemical hazards
Chlorides
Chromatography, Gas
Drug Stability
Environmental assessment
Excretion
Female
Hexanes - analysis
Hexanones - urine
Humans
Hydrogen-Ion Concentration
Hydrolysis
Industry
Male
Medical sciences
Metabolites
Occupational Exposure
Rats
Sodium
Solvents
Temperature
Time Factors
Toxicology
Urine
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Summary:2,5-Hexanedione is a main metabolite of n-hexane and is considered as the cause of n-hexane polyneuropathy. Therefore, it is useful to measure 2,5-hexanedione for biological monitoring of exposure to n-hexane. The analytical methods existing for n-hexane metabolites, however, were controversial and not established enough. Hence, a simple and precise method for determination of urinary 2,5-hexanedione has been developed. Five ml of urine was acidified to pH 0.5 with concentrated hydrochloric acid and heated for 30 minutes at 90-100 degrees C. After cooling in water, sodium chloride and dichloromethane containing internal standard were added. The sample was shaken and centrifuged. 2,5-Hexanedione concentration in an aliquot of dichloromethane extract was quantified by gas chromatography using a widebore column (DB-1701). Urinary concentration of 2,5-hexanedione showed a good correlation with exposure to n-hexane (n = 50, r = 0.973, p less than 0.001). This method is simple and precise for analysis of urinary 2,5-hexanedione as an index of exposure to n-hexane.
ISSN:0007-1072
1351-0711
1470-7926
DOI:10.1136/oem.48.8.568