Anti-integrin αvβ6 autoantibodies in patients with primary sclerosing cholangitis

Background Patients with primary sclerosing cholangitis (PSC) possess autoantibodies against biliary epithelial cells. However, the target molecules remain unknown. Methods The sera of patients with PSC and controls were subjected to enzyme-linked immunosorbent assays to detect autoantibodies using...

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Bibliographic Details
Published in:Journal of gastroenterology 2023-08, Vol.58 (8), p.778-789
Main Authors: Yoshida, Hiroyuki, Shiokawa, Masahiro, Kuwada, Takeshi, Muramoto, Yuya, Ota, Sakiko, Nishikawa, Yoshihiro, Maeda, Hirona, Kakiuchi, Nobuyuki, Okamoto, Kanako, Yamazaki, Hajime, Yokode, Masataka, Nakamura, Takeharu, Matsumoto, Shimpei, Hirano, Tomonori, Okada, Hirokazu, Marui, Saiko, Sogabe, Yuko, Matsumori, Tomoaki, Mima, Atsushi, Uza, Norimitsu, Eso, Yuji, Takai, Atsushi, Takahashi, Ken, Ueda, Yoshihide, Kodama, Yuzo, Chiba, Tsutomu, Seno, Hiroshi
Format: Article
Language:English
Subjects:
Abdominal Surgery
Autoantibodies
Bile ducts
Biliary Tract
Cholangitis
Cholangitis, Sclerosing
Colorectal Surgery
Enzyme-Linked Immunosorbent Assay
Epithelial cells
Epithelial Cells - metabolism
Fibronectin
Gastroenterology
Hepatology
Humans
Immunofluorescence
Immunoglobulins
Inflammatory Bowel Diseases
Medicine
Medicine & Public Health
Original Article―Liver
Original ―Liver, Pancreas, and Biliary Tract
Pancreas
Surgical Oncology
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Summary:Background Patients with primary sclerosing cholangitis (PSC) possess autoantibodies against biliary epithelial cells. However, the target molecules remain unknown. Methods The sera of patients with PSC and controls were subjected to enzyme-linked immunosorbent assays to detect autoantibodies using recombinant integrin proteins. Integrin αvβ6 expression in the bile duct tissues was examined using immunofluorescence. The blocking activity of the autoantibodies was examined using solid-phase binding assays. Results Anti-integrin αvβ6 antibodies were detected in 49/55 (89.1%) patients with PSC and 5/150 (3.3%) controls ( P  
ISSN:0944-1174
1435-5922
DOI:10.1007/s00535-023-02006-6