Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity

• Published in: Proceedings of the National Academy of Sciences - PNAS 2023-07, Vol.120 (30), p.e2308010120
• Main Authors: Wan, Li, Toland, Sabrina, Robinson-McCarthy, Lindsey R, Lee, Nara, Schaich, Matthew A, Hengel, Sarah R, Li, Xiaochen, Bernstein, Kara A, Van Houten, Bennett, Chang, Yuan, Moore, Patrick S
• Format: Article
• Language: English
• Subjects: Adenylyl Imidodiphosphate; Antigen T (large); Antigens, Polyomavirus Transforming - genetics; Antigens, Polyomavirus Transforming - metabolism; Assembly; ATP; Biological Sciences; Cell cycle; Deoxyribonucleic acid; DNA; DNA - genetics; DNA - metabolism; DNA helicase; DNA Helicases - genetics; DNA Helicases - metabolism; DNA Replication; DNA viruses; DNA, Single-Stranded; DNA, Viral - genetics; DNA, Viral - metabolism; Hexamers; Melting; Nuclease; Nucleic Acid Denaturation; Origins; Physical Sciences; Proteins; Replication; Replication initiation; Simian virus 40 - genetics; Simian virus 40 - metabolism; Single-stranded DNA
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2308010120

Cellular eukaryotic replication initiation helicases are first loaded as head-to-head double hexamers on double-stranded (ds) DNA origins and then initiate S-phase DNA melting during licensed (once per cell cycle) replication. Merkel cell polyomavirus (MCV) large T (LT) helicase oncoprotein similarly binds and melts its own 98-bp origin but replicates multiple times in a single cell cycle. To examine the actions of this unlicensed viral helicase, we quantitated multimerization of MCV LT molecules as they assembled on MCV DNA origins using real-time single-molecule microscopy. MCV LT formed highly stable double hexamers having 17-fold longer mean lifetime (τ, >1,500 s) on DNA than single hexamers. Unexpectedly, partial MCV LT assembly without double-hexamer formation was sufficient to melt origin dsDNA as measured by RAD51, RPA70, or S1 nuclease cobinding. DNA melting also occurred with truncated MCV LT proteins lacking the helicase domain, but was lost from a protein without the multimerization domain that could bind only as a monomer to DNA. SV40 polyomavirus LT also multimerized to the MCV origin without forming a functional hexamer but still melted origin DNA. MCV origin melting did not require ATP hydrolysis and occurred for both MCV and SV40 LT proteins using the nonhydrolyzable ATP analog, adenylyl-imidodiphosphate (AMP-PNP). LT double hexamers formed in AMP-PNP, and melted DNA, consistent with direct LT hexamer assembly around single-stranded (ss) DNA without the energy-dependent dsDNA-to-ssDNA melting and remodeling steps used by cellular helicases. These results indicate that LT multimerization rather than helicase activity is required for origin DNA melting during unlicensed virus replication.