Assessment of a 60-Biomarker Health Surveillance Panel (HSP) on Whole Blood from Remote Sampling Devices by Targeted LC/MRM-MS and Discovery DIA-MS Analysis

Telehealth, accessing healthcare and wellness remotely, should be a cost-effective and efficient way for individuals to receive care. The convenience of having a reliable remote collection device for blood tests will facilitate access to precision medicine and healthcare. Herein, we tested a 60-biom...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-07, Vol.95 (29), p.11007-11018
Main Authors: Whelan, Stephen A., Hendricks, Nathan, Dwight, Zachary L., Fu, Qin, Moradian, Annie, Van Eyk, Jennifer E., Mockus, Susan M.
Format: Article
Language:English
Subjects:
Biomarkers
Blood
Blood Specimen Collection
Chemistry
Chromatography, Liquid - methods
Cluster analysis
Clustering
Coefficient of variation
Cost analysis
Health care
Health surveillance
Heat shock proteins
Humans
Libraries
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
Mass spectroscopy
Peptides
Peptides - chemistry
Plasma
Precision medicine
Proteins
Proteomes
Samplers
Sampling
Scientific imaging
Statistical analysis
Vector quantization
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Summary:Telehealth, accessing healthcare and wellness remotely, should be a cost-effective and efficient way for individuals to receive care. The convenience of having a reliable remote collection device for blood tests will facilitate access to precision medicine and healthcare. Herein, we tested a 60-biomarker health surveillance panel (HSP), containing 35 FDA/LDT assays and covering at least 14 pathological states, on 8 healthy individuals’ ability to collect their own capillary blood from a lancet finger prick and directly compared it to the traditional phlebotomist venous blood and plasma collection methods. All samples were spiked with 114 stable-isotope-labeled (SIL) HSP peptides and quantitatively analyzed by liquid chromatography-multiple reaction monitoring-mass spectrometry (LC/MRM-MS) scheduled method targeting 466 transitions from 114 HSP peptides and by a discovery data-independent acquisition mass spectrometry (DIA-MS) method. The average peak area ratio (PAR) of the HSP quantifier peptide transitions from all 8 volunteers’ capillary blood (n = 48), venous blood (n = 48), and matched plasma (n = 24) was 90% similarity. Discovery DIA-MS analysis of the same samples using a plasma spectral library and a pan-human spectral library identified 1121 and 4661 total proteins, respectively. In addition, at least 122 FDA-approved biomarkers were identified. DIA-MS analysis reproducibly quantitated (
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.3c01189