Multi-Drug Resistance in Bacterial Genomes-A Comprehensive Bioinformatic Analysis

Antimicrobial resistance is presently one of the greatest threats to public health. The excessive and indiscriminate use of antibiotics imposes a continuous selective pressure that triggers the emergence of multi-drug resistance. We performed a large-scale analysis of closed bacterial genomes to ide...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-07, Vol.24 (14), p.11438
Main Authors: Domingues, Célia P F, Rebelo, João S, Dionisio, Francisco, Nogueira, Teresa
Format: Article
Language:English
Subjects:
Analysis
Anti-Bacterial Agents - pharmacology
Antibiotics
Bacteria
Bacterial genetics
Bacterial infections
Computational Biology
Datasets
Drug resistance
Drug resistance in microorganisms
Drug Resistance, Bacterial - genetics
Drug Resistance, Multiple
Drug Resistance, Multiple, Bacterial - genetics
Expected values
Genes
Genome, Bacterial
Genomes
Genomics
Health aspects
Heavy metals
Plasmids
Plasmids - genetics
Proteins
Public health
Tetracycline
Tetracyclines
Virulence
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Summary:Antimicrobial resistance is presently one of the greatest threats to public health. The excessive and indiscriminate use of antibiotics imposes a continuous selective pressure that triggers the emergence of multi-drug resistance. We performed a large-scale analysis of closed bacterial genomes to identify multi-drug resistance considering the ResFinder antimicrobial classes. We found that more than 95% of the genomes harbor genes associated with resistance to disinfectants, glycopeptides, macrolides, and tetracyclines. On average, each genome encodes resistance to more than nine different classes of antimicrobial drugs. We found higher-than-expected co-occurrences of resistance genes in both plasmids and chromosomes for several classes of antibiotic resistance, including classes categorized as critical according to the World Health Organization (WHO). As a result of antibiotic-resistant priority pathogens, higher-than-expected co-occurrences appear in plasmids, increasing the potential for resistance dissemination. For the first time, co-occurrences of antibiotic resistance have been investigated for priority pathogens as defined by the WHO. For critically important pathogens, co-occurrences appear in plasmids, not in chromosomes, suggesting that the resistances may be epidemic and probably recent. These results hint at the need for new approaches to treating infections caused by critically important bacteria.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241411438