Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model

• Published in: Journal of parasitic diseases 2023-09, Vol.47 (3), p.520-526
• Main Authors: Corrêa, Luisa Queiroz, do Couto, Bruna Patrícia, de Carvalho, Edson Fernando Goulart, de Sousa, José Eduardo Neto, da Silva Ribeiro, Vanessa, Gonzaga, Henrique Tomaz, Costa-Cruz, Julia Maria
• Format: Article
• Language: English
• Subjects: Animal models; detection limit; Dexamethasone; eggs; feces; Health Promotion and Disease Prevention; Immunoglobulin G; Immunosuppression; Infectious Diseases; Inoculation; Medicine; Medicine & Public Health; Oral administration; Original; Original Article; parasite load; Parasites; phosphates; Rattus norvegicus; Strongyloides; Strongyloides venezuelensis; Strongyloidiasis; subcutaneous injection
• ISSN: 0971-7196; 0975-0703
• DOI: 10.1007/s12639-023-01595-6

Rodents infected with Strongyloides venezuelensis are experimental models applied to strongyloidiasis research. This study evaluated oral and subcutaneous dexamethasone (DEX) treatments to establish immunosuppression in an experimental model of Strongyloides hyperinfection. Rattus norvegicus Wistar were divided: G I (−): untreated and uninfected animals, G II (+): untreated and infected, G III (o −) orally treated and uninfected, G IV (o +) orally treated and infected, G V (sc −) subcutaneously treated and uninfected, G VI (sc +) subcutaneously treated and infected. For oral administration, DEX was diluted in sterile water (5 µg/ml) and made available to the animals on intervals in experimental days − 5–0, 8–13 and 21–26. For subcutaneous administration, animals received daily injections of DEX disodium phosphate (2 mg/kg). Infection was established by the subcutaneous inoculation of 3000 S. venezuelensis filarioid larvae. Groups were evaluated by egg per gram of feces and parasite females counts and IgG, IgG1 and IgG2a detection. GIV (o +) had egg peaks count on days 13 and 26 and maintained egg elimination until the last experimental day. Parasitic females recovery at day 30 was significantly higher in G IV (o +) when compared to G VI (sc +). Levels of IgG, IgG1 and IgG2a of all groups, except the positive control GII (+), were below the detection threshold. Pharmacological immunosuppression induced by oral administration of DEX produced high parasitic burden, and is a noninvasive method, useful to establish immunosuppression in strongyloidiasis hyperinfection model in rats.