Plasma proteomic profiles of pain subtypes in adolescents and young adults with endometriosis

• Published in: Human reproduction (Oxford) 2023-08, Vol.38 (8), p.1509-1519
• Main Authors: Sasamoto, Naoko, Ngo, Long, Vitonis, Allison F, Dillon, Simon T, Sieberg, Christine B, Missmer, Stacey A, Libermann, Towia A, Terry, Kathryn L
• Format: Article
• Language: English
• Subjects: Abdominal Pain; Cross-Sectional Studies; Dysmenorrhea; Endometriosis - diagnosis; Female; Humans; Original; Pelvic Pain - diagnosis; Proteomics
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/humrep/dead099

Abstract STUDY QUESTION What are the similarities and differences in the systemic proteomic profiles by endometriosis-associated pain subtypes among adolescents and young adults with endometriosis? SUMMARY ANSWER Endometriosis-associated pain subtypes exhibited distinct plasma proteomic profiles. WHAT IS KNOWN ALREADY Endometriosis patients, especially those diagnosed in adolescents and young adults, are often plagued by various pain symptoms. However, it is not clear what biological processes underlie this heterogeneity. STUDY DESIGN, SIZE, DURATION We conducted a cross-sectional analysis using data and plasma samples from 142 adolescent or young adult participants of the Women’s Health Study: From Adolescence to Adulthood cohort with laparoscopically confirmed endometriosis. PARTICIPANTS/MATERIALS, SETTING, METHODS We measured 1305 plasma protein levels by SomaScan. We classified self-reported endometriosis-associated pain into subtypes of dysmenorrhea, acyclic pelvic pain, life impacting pelvic pain, bladder pain, bowel pain, and widespread pain phenotype. We used logistic regression to calculate the odds ratios and 95% confidence intervals for differentially expressed proteins, adjusting for age, BMI, fasting status, and hormone use at blood draw. Ingenuity Pathway Analysis identified enriched biological pathways. MAIN RESULTS AND THE ROLE OF CHANCE Our study population consisted mainly of adolescents and young adults (mean age at blood draw = 18 years), with nearly all (97%) scored as rASRM stage I/II at laparoscopic diagnosis of endometriosis, which is a common clinical presentation of endometriosis diagnosed at a younger age. Pain subtypes exhibited distinct plasma proteomic profiles. Multiple cell movement pathways were downregulated in cases with severe dysmenorrhea and life impacting pelvic pain compared to those without (P