Impact of 1,6-hexanediol on Schizosaccharomyces pombe genome stability

Abstract Phase separation is a major mechanism of macromolecular condensation within cells. A frequently chosen tool for global disruption of phase separation via weak hydrophobic interactions is treatment with 1,6-hexanediol. This study evaluates the cytotoxic and genotoxic effects of treating live...

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Bibliographic Details
Published in:G3 : genes - genomes - genetics 2023-08, Vol.13 (8)
Main Authors: Jones, Chance E, Forsburg, Susan L
Format: Article
Language:English
Subjects:
Genomic Instability
Heterochromatin - metabolism
Humans
Investigation
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe Proteins - genetics
Schizosaccharomyces pombe Proteins - metabolism
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Summary:Abstract Phase separation is a major mechanism of macromolecular condensation within cells. A frequently chosen tool for global disruption of phase separation via weak hydrophobic interactions is treatment with 1,6-hexanediol. This study evaluates the cytotoxic and genotoxic effects of treating live fission yeast with 1,6-hexanediol. We find that 1,6-hexanediol causes a drastic decrease in cell survival and growth rate. We also see a reduction in HP1 protein foci and increase in DNA damage foci. However, there is no evidence for increased genomic instability in two classically phase-separated domains, the heterochromatic pericentromere and the nucleolar rDNA repeats. This study reveals that 1,6-hexanediol is a blunt tool for phase separation inhibition and its secondary effects must be taken into consideration during its in vivo use.
ISSN:2160-1836
2160-1836
DOI:10.1093/g3journal/jkad123