Effects of cyclophosphamide on rat placental development

We examined the morphological effects of cyclophosphamide (CPA) on placental development in pregnant rats. CPA was administered as a single dose to pregnant rats intraperitoneally at 0 mg/kg (the control group), 25 mg/kg on gestation day (GD) 12 (the CPA GD 12-treated group), and 25 mg/kg on GD 14 (...

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Bibliographic Details
Published in:Journal of Toxicologic Pathology 2023, Vol.36(3), pp.159-169
Main Authors: Furukawa, Satoshi, Tsuji, Naho, Hayashi, Seigo, Kuroda, Yusuke, Kimura, Masayuki, Kojima, Chisato, Takeuchi, Kazuya
Format: Article
Language:English
Subjects:
Apoptosis
Cell proliferation
Cyclophosphamide
Fetal resorption
Fetuses
Glands
Glycogen
Glycogens
Hypoplasia
Natural killer cells
Original
Placenta
rat
small placenta
syncytiotrophoblast
Trophoblasts
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Summary:We examined the morphological effects of cyclophosphamide (CPA) on placental development in pregnant rats. CPA was administered as a single dose to pregnant rats intraperitoneally at 0 mg/kg (the control group), 25 mg/kg on gestation day (GD) 12 (the CPA GD 12-treated group), and 25 mg/kg on GD 14 (the CPA GD 14-treated group). The fetal and placental weight decreased in the CPA-treated groups, complete fetal resorption from GD 17 onwards in the CPA GD 12-treated group, and external malformations in the CPA GD 14-treated group. Histopathologically, CPA induced apoptosis and/or cell proliferation inhibition in each part of the placenta. In the labyrinth zone, syncytiotrophoblasts were selectively reduced, resulting in a small placenta. In the basal zone, the number of spongiotrophoblasts was reduced, resulting in hypoplasia of glycogen cell islands. In addition, a small number of interstitial trophoblasts invaded the metrial gland from the basal zone on GD 15. The severity of these lesions was higher in the CPA GD 12-treated group than in the CPA GD 14-treated group. In the metrial gland, although the number of uterine natural killer cells was reduced, metrial gland development was not affected.
ISSN:0914-9198
1881-915X
1347-7404
DOI:10.1293/tox.2022-0144