UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination and DNA repair

The ubiquitin signaling pathway is crucial for the DNA damage response pathway. More specifically, RNF168 is integral in regulating DNA repair proteins at damaged chromatin. However, the detailed mechanism by which RNF168 is regulated in cells is not fully understood. Here, we identify the ubiquitin...

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Bibliographic Details
Published in:The Journal of biological chemistry 2023-08, Vol.299 (8), p.105043-105043, Article 105043
Main Authors: Lee, Seong-Ok, Kelliher, Jessica L., Song, Wan, Tengler, Kyle, Sarkar, Aradhan, Dray, Eloise, Leung, Justin W.C.
Format: Article
Language:English
Subjects:
53BP1
acidic patch
DNA Damage
DNA Repair
Histones - metabolism
Humans
nucleosome
Nucleosomes - genetics
ribosomal proteins
Ribosomal Proteins - metabolism
Ubiquitin - metabolism
Ubiquitin-Protein Ligases - metabolism
Ubiquitination
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Summary:The ubiquitin signaling pathway is crucial for the DNA damage response pathway. More specifically, RNF168 is integral in regulating DNA repair proteins at damaged chromatin. However, the detailed mechanism by which RNF168 is regulated in cells is not fully understood. Here, we identify the ubiquitin-ribosomal fusion proteins UBA80 (also known as RPS27A) and UBA52 (also known as RPL40) as interacting proteins for H2A/H2AX histones and RNF168. Both UBA80 and UBA52 are recruited to laser-induced micro-irradiation DNA damage sites and are required for DNA repair. Ectopic expression of UBA80 and UBA52 inhibits RNF168-mediated H2A/H2AX ubiquitination at K13/15 and impairs 53BP1 recruitment to DNA lesions. Mechanistically, the C-terminal ribosomal fragments of UBA80 and UBA52, S27A and L40, respectively, limit RNF168-nucleosome engagement by masking the regulatory acidic residues at E143/E144 and the nucleosome acidic patch. Together, our results reveal that UBA80 and UBA52 antagonize the ubiquitination signaling pathway and fine-tune the spatiotemporal regulation of DNA repair proteins at DNA damage sites.
ISSN:0021-9258
1083-351X
DOI:10.1016/j.jbc.2023.105043