K-Sol corneal preservation at room temperature

K-Sol, a recently developed corneal storage medium that contains purified chondroitin sulphate in tissue culture medium (TC 199), is capable of preserving corneal tissue for 14 days at 4 degrees C. To study the effect of tissue storage in K-Sol at room temperature we preserved rabbit corneas in K-So...

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Bibliographic Details
Published in:British journal of ophthalmology 1988-05, Vol.72 (5), p.370-376
Main Authors: Tamaki, K., Varnell, E. D., Kaufman, H. E.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chondroitin - analogs & derivatives
Chondroitin Sulfates
Cornea - ultrastructure
Corneal Transplantation
Endothelium, Corneal - ultrastructure
Gentamicins
HEPES
Humans
Hydrogen-Ion Concentration
Medical sciences
Organoids - ultrastructure
Piperazines
Rabbits
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the eye and orbit
Temperature
Time Factors
Tissue Preservation - methods
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Summary:K-Sol, a recently developed corneal storage medium that contains purified chondroitin sulphate in tissue culture medium (TC 199), is capable of preserving corneal tissue for 14 days at 4 degrees C. To study the effect of tissue storage in K-Sol at room temperature we preserved rabbit corneas in K-Sol and M-K medium for three or seven days at 25 degrees C. All of the corneal endothelial sheets were intact after three days. At seven days the change in pH of the K-Sol medium was less than that of M-K medium. Corneas preserved in M-K medium showed swelling of mitochondria and a decrease in the number of cytoplasmic organelles. Corneas preserved in K-Sol had organised cytoplasmic organelles and nuclei. Scanning electron micrographs revealed well preserved endothelial sheets. Corneas stored in the two media showed no significant difference in thickness. A pair of human corneas preserved in K-Sol at room temperature for six days maintained about 95% of the endothelial sheet in good condition. Small separations were observed between some of the endothelial cells. However, even in these areas, the cytoplasmic organelles were well preserved. It appeared that K-Sol is more stable than M-K medium at room temperature, and that both rabbit and human corneas can be preserved in good condition in K-Sol for at least six days at 25 degrees C.
ISSN:0007-1161
1468-2079
DOI:10.1136/bjo.72.5.370