Establishment and Characterization of Mild Atopic Dermatitis in the DNCB-Induced Mouse Model

In dermatological research, 2,4-dinitrochlorbenzene (DNCB)-induced atopic dermatitis (AD) is a standard model as it displays many disease-associated characteristics of human AD. However, the reproducibility of the model is challenging due to the lack of information regarding the methodology and the...

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Published in:International journal of molecular sciences 2023-08, Vol.24 (15), p.12325
Main Authors: Riedl, Rebecca, Kühn, Annika, Rietz, Denise, Hebecker, Betty, Glowalla, Karl-Gunther, Peltner, Lukas K, Jordan, Paul M, Werz, Oliver, Lorkowski, Stefan, Wiegand, Cornelia, Wallert, Maria
Format: Article
Language:English
Subjects:
Advertising executives
Analysis
Animals
Atopic dermatitis
Cytokeratin
Cytokines
Dermatitis
Eczema
Experiments
Gene expression
Histamine
Hyperplasia
Immunoglobulin E
Lymphatic system
Medical research
Medicine, Experimental
Morphology
Pharmaceutical industry
Polyunsaturated fatty acids
Proteins
Skin
Skin diseases
Unsaturated fatty acids
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Summary:In dermatological research, 2,4-dinitrochlorbenzene (DNCB)-induced atopic dermatitis (AD) is a standard model as it displays many disease-associated characteristics of human AD. However, the reproducibility of the model is challenging due to the lack of information regarding the methodology and the description of the phenotype and endotype of the mimicked disease. In this study, a DNCB-induced mouse model was established with a detailed procedure description and classification of the AD human-like skin type. The disease was induced with 1% DNCB in the sensitization phase and repeated applications of 0.3% and 0.5% DNCB in the challenging phase which led to a mild phenotype of AD eczema. Pathophysiological changes of the dorsal skin were measured: thickening of the epidermis and dermis, altered skin barrier proteins, increased TH1 and TH2 cytokine expression, a shift in polyunsaturated fatty acids, increased pro-resolving and inflammatory mediator formation, and dysregulated inflammation-associated gene expression. A link to type I allergy reactions was evaluated by increased mast cell infiltration into the skin accompanied by elevated IgE and histamine levels in plasma. As expected for mild AD, no systemic inflammation was observed. In conclusion, this experimental setup demonstrates many features of a mild human-like extrinsic AD in murine skin.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241512325