Protective role of melatonin against testicular damage caused by polymicrobial sepsis in adult rats

This study aimed to investigate the possible protective effects of melatonin (MEL) against the damage to testicular tissue in rats caused by polymicrobial sepsis as a result of cecal ligation and perforation (CLP). In this study, 21 male Wistar albino rats were used. The rats were randomly divided i...

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Published in:Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES 2022-06, Vol.28 (6), p.723-729
Main Authors: Doğanay, Songül, Budak, Özcan, Toprak, Veysel, Erman, Gülay, Şahin, Arzu
Format: Article
Language:English
Subjects:
Experimental Study
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Summary:This study aimed to investigate the possible protective effects of melatonin (MEL) against the damage to testicular tissue in rats caused by polymicrobial sepsis as a result of cecal ligation and perforation (CLP). In this study, 21 male Wistar albino rats were used. The rats were randomly divided into three groups (n=7): Sham Control (Group 1), CLP (Group 2), and CLP + MEL (Group 3). Sepsis was created using the CLP method. MEL was administered intraperitoneally in two equal doses of 10 mg/kg at 30 min before and 6 h after perforation. Tissue sections taken from paraffin blocks were stained with hematoxylin and eosin (H and E) and examined histopathologically under a light microscope. Intracellular H2O2 and apoptosis evaluations were carried out using the flow cytometric method. Sepsis caused a significant reduction in all sperm parameters. There was a significant decrease in sperm density, motility and cell numbers with normal morphology (p
ISSN:1306-696X
1307-7945
DOI:10.14744/tjtes.2021.90575