RNA sequencing identified novel target genes for Adansonia digitata in breast and colon cancer cells

Adansonia digitata exhibits numerous beneficial effects. In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7),...

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Bibliographic Details
Published in:Science progress (1916) 2021-07, Vol.104 (3), p.368504211032084-368504211032084
Main Authors: El-Masry, Omar S., Goja, Arafat, Rateb, Mostafa, Owaidah, Amani Y, Alsamman, Khaldoon
Format: Article
Language:English
Subjects:
Adansonia
Adansonia digitata
Anticancer properties
Apoptosis
Breast cancer
Casein
Casein Kinase II
Cell Line, Tumor
Colon
Colon cancer
Colonic Neoplasms - drug therapy
Colonic Neoplasms - genetics
Colorectal cancer
Female
Fibers
Gene expression
Gene sequencing
Genes
Humans
Kinases
Ovarian cancer
Ovarian Neoplasms
Plant Extracts
Ribonucleic acid
RNA
Sequence Analysis, RNA
Tumor cell lines
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Summary:Adansonia digitata exhibits numerous beneficial effects. In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7), and human ovarian cancer (OVCAR-3 and OVCAR-4) cell lines. RNA sequencing revealed the influence of the effective A. digitata fraction on the gene expression profiles of responsive cells. The results indicated that only the polar extract of the A. digitata fibers exhibited anti-proliferative activities against HCT116 and MCF-7 cells, but not ovarian cancer cells. Moreover, the polar extract of the fibers resulted in the modulation of the expression of multiple genes in HCT116 and MCF-7 cells. We propose that casein kinase 2 alpha 3 (CSNK2A3) is a novel casein kinase 2 (CSNK2) isoform in HCT116 cells and report, for the first time, the potential involvement of FYVE, RhoGEF, and PH domain-containing 3 (FGD3) in colon cancer. Together, these findings provide evidence supporting the anti-cancer potential of the polar extract of A. digitata fibers in this experimental model of breast and colon cancers.
ISSN:0036-8504
2047-7163
DOI:10.1177/00368504211032084