Proteomic and Mutant Analysis of Hydrogenase Maturation Protein Gene hypE in Symbiotic Nitrogen Fixation of Mesorhizobium huakuii

Hydrogenases catalyze the simple yet important redox reaction between protons and electrons and H2, thus mediating symbiotic interactions. The contribution of hydrogenase to this symbiosis and anti-oxidative damage was investigated using the M. huakuii hypE (encoding hydrogenase maturation protein)...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-08, Vol.24 (16), p.12534
Main Authors: Long, Songhua, Su, Min, Chen, Xiaohong, Hu, Aiqi, Yu, Fuyan, Zou, Qian, Cheng, Guojun
Format: Article
Language:English
Subjects:
Bacteria
Biosynthesis
E coli
Hydrogen
Ligands
Nitrogen
Proteins
Scanning electron microscopy
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Summary:Hydrogenases catalyze the simple yet important redox reaction between protons and electrons and H2, thus mediating symbiotic interactions. The contribution of hydrogenase to this symbiosis and anti-oxidative damage was investigated using the M. huakuii hypE (encoding hydrogenase maturation protein) mutant. The hypE mutant grew a little faster than its parental 7653R and displayed decreased antioxidative capacity under H2O2-induced oxidative damage. Real-time quantitative PCR showed that hypE gene expression is significantly up-regulated in all the detected stages of nodule development. Although the hypE mutant can form nodules, the symbiotic ability was severely impaired, which led to an abnormal nodulation phenotype coupled to a 47% reduction in nitrogen fixation capacity. This phenotype was linked to the formation of smaller abnormal nodules containing disintegrating and prematurely senescent bacteroids. Proteomics analysis allowed a total of ninety differentially expressed proteins (fold change > 1.5 or
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241612534