A Two-Mediator System Based on a Nanocomposite of Redox-Active Polymer Poly(thionine) and SWCNT as an Effective Electron Carrier for Eukaryotic Microorganisms in Biosensor Analyzers

Electropolymerized thionine was used as a redox-active polymer to create a two-mediated microbial biosensor for determining biochemical oxygen demand (BOD). The electrochemical characteristics of the conducting system were studied by cyclic voltammetry and electrochemical impedance spectroscopy. It...

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Published in:Polymers 2023-08, Vol.15 (16), p.3335
Main Authors: Medvedeva, Anastasia S., Dyakova, Elena I., Kuznetsova, Lyubov S., Mironov, Vladislav G., Gurkin, George K., Rogova, Tatiana V., Kharkova, Anna S., Melnikov, Pavel V., Naumova, Alina O., Butusov, Denis N., Arlyapov, Vyacheslav A.
Format: Article
Language:English
Subjects:
Analyzers
Bacteria
Biochemical oxygen demand
Biosensors
Composite materials
Detectors
Dilution
Electrochemical impedance spectroscopy
Electrodes
Electron transfer
Enzymes
Eukaryotes
Glucose
Graphene
Graphite
Microorganisms
Nanocomposites
Nanomaterials
Polymer industry
Polymerization
Polymers
Potassium
Single wall carbon nanotubes
Yeast
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Summary:Electropolymerized thionine was used as a redox-active polymer to create a two-mediated microbial biosensor for determining biochemical oxygen demand (BOD). The electrochemical characteristics of the conducting system were studied by cyclic voltammetry and electrochemical impedance spectroscopy. It has been shown that the most promising in terms of the rate of interaction with the yeast B. adeninivorans is the system based on poly(thionine), single-walled carbon nanotubes (SWCNT), and neutral red (kint = 0.071 dm3/(g·s)). The biosensor based on this system is characterized by high sensitivity (the lower limit of determined BOD concentrations is 0.4 mgO2/dm3). Sample analysis by means of the developed analytical system showed that the results of the standard dilution method and those using the biosensor differed insignificantly. Thus, for the first time, the fundamental possibility of effectively using nanocomposite materials based on SWCNT and the redox-active polymer poly(thionine) as one of the components of two-mediator systems for electron transfer from yeast microorganisms to the electrode has been shown. It opens up prospects for creating stable and highly sensitive electrochemical systems based on eukaryotes.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym15163335