A wound inducible chalcone synthase gene from Dysoxylum gotadhora (DbCHS) regulates flavonoid biosynthesis

• Published in: Physiology and molecular biology of plants 2023-07, Vol.29 (7), p.959-969
• Main Authors: Mahajan, Vidushi, Chouhan, Rekha, Jamwal, Vijay Lakshmi, Kapoor, Nitika, Gandhi, Sumit G.
• Format: Article
• Language: English
• Subjects: Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biosynthesis; Cell Biology; Chalcone synthase; CHS gene; Cloning; Enzymes; Flavonoids; Immunomodulation; Inflammation; Leaves; Life Sciences; Microorganisms; Molecular docking; Open reading frames; Phylogeny; Plant Physiology; Plant Sciences; Plant species; Plants; Polyketide synthase; Research Article; Seeds; Wounding
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-023-01344-2

Chalcone synthase (CHS) is a type III polyketide synthase and a key enzyme of the phenylpropanoid pathway that generates precursors for flavonoid biosynthesis. The tree species D. gotadhora is known for having an abundance of rohitukine, which has anti-inflammatory and immune-modulating effects. In this study, we used the leaves of D. gotadhora to clone CHS gene ( DbCHS ). The 1188-bp open reading frame (ORF) was part of the 1373-bp full-length DbCHS clone. Compared to other parts of the plant, DbCHS is expressed more in the leaves and fruits. This is linked to anti-microbial action against a panel of microbes in these tissues. The leaves and seeds extracts inhibit Bacillus subtilis , Streptococcus pyogenes , Bacillus cereus , and Candida albicans . When a plant is hurt, it leaves its tissues open to attack by microbes. To protect themselves, plants often make chemicals that kill microbes. We found that wounding had a big effect on the production of DbCHS. Based on these tests and the results of phylogenetic analysis and molecular docking, we believe that DbCHS is a wound-inducible enzyme that is needed to make flavonoids, which may give the plant antimicrobial properties.