Nematode predatory ability of the fungus Duddingtonia flagrans affected by in vitro sequential exposure to ovine gastrointestinal tract

Duddingtonia flagrans is a nematophagous fungus employed as a biocontrol agent of gastrointestinal nematodes in ruminants. After oral ingestion and passage through the digestive tract of animals, this microorganism captures the nematodes in the feces. The drastic conditions of ruminant digestive tra...

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Bibliographic Details
Published in:Veterinary research communications 2023-09, Vol.47 (3), p.1405-1412
Main Authors: Céspedes-Gutiérrez, Elizabeth, Aragón, Diana Marcela, Gómez-Álvarez, Martha Isabel, Cubides-Cárdenas, Jaime Andrés, Cortés-Rojas, Diego Francisco
Format: Article
Language:English
Subjects:
Abomasum
Anaerobiosis
Biological control
Biomedical and Life Sciences
Chlamydospores
Duddingtonia flagrans
Fungi
Gastrointestinal tract
Life Sciences
Nematodes
Nematophagous fungi
Oral cavity
Pancreatin
Pepsin
Small intestine
Veterinary Medicine/Veterinary Science
Zoology
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Summary:Duddingtonia flagrans is a nematophagous fungus employed as a biocontrol agent of gastrointestinal nematodes in ruminants. After oral ingestion and passage through the digestive tract of animals, this microorganism captures the nematodes in the feces. The drastic conditions of ruminant digestive tract could affect fungi chlamydospores and therefore biocontrol activity. The aim of this study was to evaluate in vitro the effect of four ruminant digestive segments on the concentration and nematode predatory ability of a Colombian native strain of D. flagrans . The sequential four-step methodology proposed evaluated conditions of the oral cavity, rumen, abomasum, and small intestine such as pH (2, 6, 8), enzymes (pepsin, pancreatin), temperature (39 °C), and anaerobiosis comparing short (7 h) and long (51 h) exposure times. The results showed that the nematode predatory ability of the fungi is affected by sequential exposure to gastrointestinal segments and this effect depends on the exposure time to those conditions. After short exposure (7 h) through the four ruminant digestive segments, the fungi had a nematode predatory ability of 62%, in contrast, after long exposure (51 h) the nematode predatory ability was lost (0%). Moreover, the number of broken chlamydospores was higher in the long-exposure assay.
ISSN:0165-7380
1573-7446
DOI:10.1007/s11259-023-10089-y