Comparing the biological properties and chemical profiling of chestnut bee pollen and bee bread collected from Anatolia

• Published in: Brazilian journal of microbiology 2023-09, Vol.54 (3), p.2307-2317
• Main Authors: Sonmez, Emine, Kekecoglu, Meral, Sahin, Huseyin, Bozdeveci, Arif, Karaoglu, Sengul ALPAY
• Format: Article
• Language: English
• Subjects: Animals; Anti-Infective Agents - chemistry; Antimicrobial activity; Antimicrobial agents; Antioxidants; Antioxidants - analysis; Antioxidants - pharmacology; Bacteria; Bee bread; Bees; Biological properties; Biomedical and Life Sciences; Flavonoids; Food Microbiology; Functional foods & nutraceuticals; Gram-negative bacteria; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Minimum inhibitory concentration; Mycology; p-Coumaric acid; Phenolic compounds; Phenols; Phenols - chemistry; Pollen; Propolis - chemistry; Propolis - pharmacology; Rutin; Soil and Agricultural Microbiology - Research Paper; Turkey
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-023-00980-w

Bee bread (BB) and bee pollen (BP) are accepted as functional food and considered in medical properties due to its important bioactive components. These bee products show different biological properties, but researches on these aspects have not been clear yet. In present study, Anatolian BB and BP extracts were analyzed for the first time for their pollen type, total phenolic (TPC) and flavonoid content (TFC), and antimicrobial and antioxidant properties. Samples were analyzed for their antimicrobial efficacy by the agar well diffusion and MIC methods. HPLC analysis was used to identify the compounds in the BB and BP samples. Antioxidant activity was measured by the FRAP and DPPH methods. As a result of microscopy for pollen identification, Fagaceae family was dominant. Phenolic compound analysis showed that the amounts of p -coumaric acid and rutin were found to be the highest in BB and BP, respectively. Stronger antioxidant activity was obtained from BP. MIC values of BB were range from 250 to 12.5 μg/mL. The most susceptible bacterium was Mycobacterium smegmatis . The extract of BP was effective on all gram-negative bacteria with doses range from 250 μg/mL to 500 μg/mL. The lowest MIC value was detected with the concentration of 12.5 μg/mL against M. smegmatis . Anatolian BB and BP could be considered as a functional foods due to antioxidant activity and may be beneficial in the management and treatment of pathogenic bacteria because of high antimicrobial activity.