Characterization of avian pathogenic Escherichia coli isolates based on biofilm formation, ESBL production, virulence-associated genes, and phylogenetic groups

• Published in: Brazilian journal of microbiology 2023-09, Vol.54 (3), p.2413-2425
• Main Authors: Borges, Karen Apellanis, Furian, Thales Quedi, de Brito, Benito Guimarães, de Brito, Kelly Cristina Tagliari, da Rocha, Daniela Tonini, Salle, Carlos Tadeu Pippi, Moraes, Hamilton Luiz de Souza, do Nascimento, Vladimir Pinheiro
• Format: Article
• Language: English
• Subjects: Animals; Biofilms; Biomedical and Life Sciences; Birds - microbiology; Chickens - microbiology; Colibacillosis; E coli; Escherichia coli; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Food Microbiology; Genes; Humans; Hydrolases - genetics; In vivo methods and tests; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mycology; Pathogenicity; Pathogens; Phenotypes; Phylogenetics; Phylogeny; Polymerase chain reaction; Poultry Diseases - microbiology; Veterinary Microbiology - Research Paper; Virulence; Virulence - genetics; Virulence Factors - genetics; β Lactamase
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1007/s42770-023-01026-x

    Escherichia coli is a part of both animal and human commensal microbiota. Avian pathogenic E. coli (APEC) is responsible for colibacillosis in poultry, an economically important disease. However, the close similarities among APEC isolates make it difficult to differentiate between pathogenic and commensal bacteria. The aim of this study was to determine phenotypic and molecular characteristics of APEC isolates and to compare them with their in vivo pathogenicity indices. A total of 198 APEC isolates were evaluated for their biofilm-producing ability and extended-spectrum β-lactamase (ESBL) production phenotypes. In addition, 36 virulence-associated genes were detected, and the isolates were classified into seven phylogenetic groups using polymerase chain reaction. The sources of the isolates were not associated with biofilms, ESBL, genes, or phylogroups. Biofilm and ESBL production were not associated with pathogenicity. Group B2 had the highest pathogenicity index. Groups B2 and E were positively associated with high-pathogenicity isolates and negatively associated with low-pathogenicity isolates. In contrast, groups A and C were positively associated with apathogenic isolates, and group B1 was positively associated with low-pathogenicity isolates. Some virulence-associated genes showed positive or negative associations with specific phylogenetic groups. None of the individual techniques produced results that correlated with the in vivo pathogenicity index. However, the combination of two techniques, namely, detection of virulence-associated genes and the phylogenetic groups, could help the classification of the isolates as pathogenic or commensal.